Should you encounter any unexpected behaviour,
please let us know.
elNémo has been hacked on november 27th.
It has been moved away and runs again.
Still some additional cleaning from time to time
Sorry for the inconvenience.

* MEMBRANE PROTEIN 20-NOV-23 8X5Y *

B-factor analysis for ID 2601201921523260072

Overall B-factor prediction

Correlation= -0.120 for 274 C-alpha atoms.

B-factors for all residues

Residue-by-residue B-factor prediction for CA atoms

B-factors are computed based on the first 100 normal modes (B-PREDICT) and are scaled to match the overall B-factors in the submitted model (B-SCALED). A high correlation with the observations is an indicator that the normal modes capture well the overall flexibility features of the model. Comparison of the observed (B-OBS, blue) and the computed (B-SCALED, red) B-factors for all C-alpha atoms is presented below. Differences between B-factors from normal mode analysis and from X-ray crystallographic data may for example indicate regions where the protein flexibility is modified due to external factors such as tight crystall-packing.

ATOM TYP RES RESID B-PREDICT B-SCALED B-OBS	B-SCALED	B-OBS
2 CA THR 20 0.0537 148.9871 91.8600
16 CA THR 21 0.0226 113.6268 89.0500
30 CA MET 22 0.0213 112.2248 86.6600
47 CA ALA 23 0.0177 108.0469 87.7900
57 CA SER 24 0.0353 128.0970 85.4400
68 CA PRO 25 0.0372 130.1993 84.7800
82 CA GLN 26 0.0344 127.0575 81.2700
99 CA LEU 27 0.0163 106.5034 80.5400
118 CA MET 28 0.0141 104.0479 81.6100
135 CA PRO 29 0.0199 110.5627 78.2400
149 CA LEU 30 0.0105 99.8877 79.8600
168 CA VAL 31 0.0065 95.3398 81.2900
184 CA VAL 32 0.0114 100.8782 80.5300
200 CA VAL 33 0.0100 99.3937 81.9800
216 CA LEU 34 0.0043 92.8611 84.7800
235 CA SER 35 0.0050 93.6735 86.5900
246 CA THR 36 0.0066 95.4959 89.4500
260 CA ILE 37 0.0040 92.5762 89.5600
279 CA CYS 38 0.0030 91.4264 91.9100
290 CA LEU 39 0.0055 94.2343 89.6400
309 CA VAL 40 0.0041 92.6277 92.8900
325 CA THR 41 0.0027 91.0244 94.1800
339 CA VAL 42 0.0031 91.4731 91.8500
355 CA GLY 43 0.0039 92.4000 92.3100
362 CA LEU 44 0.0028 91.1130 92.6100
381 CA ASN 45 0.0024 90.7439 91.6900
395 CA LEU 46 0.0039 92.4114 89.5100
414 CA LEU 47 0.0038 92.3372 87.2400
433 CA VAL 48 0.0025 90.8545 87.4600
449 CA LEU 49 0.0030 91.3586 82.9700
468 CA TYR 50 0.0066 95.5311 79.1300
489 CA ALA 51 0.0050 93.6112 83.8300
499 CA VAL 52 0.0042 92.7123 84.8000
515 CA ARG 53 0.0071 96.0751 82.2100
539 CA SER 54 0.0100 99.2843 83.6800
550 CA GLU 55 0.0088 97.9659 83.3900
565 CA ARG 56 0.0152 105.2840 90.6500
589 CA LYS 57 0.0131 102.8408 87.3000
611 CA LEU 58 0.0052 93.8530 90.4800
630 CA HIS 59 0.0065 95.3975 93.5600
647 CA THR 60 0.0046 93.2521 94.9600
661 CA VAL 61 0.0033 91.7873 97.6700
677 CA GLY 62 0.0025 90.7693 96.2600
684 CA ASN 63 0.0023 90.5495 94.5900
698 CA LEU 64 0.0025 90.7891 94.5300
717 CA TYR 65 0.0020 90.2766 96.3600
738 CA ILE 66 0.0017 89.8857 92.5700
757 CA VAL 67 0.0020 90.2206 90.3000
773 CA SER 68 0.0018 90.0265 93.4200
784 CA LEU 69 0.0015 89.7410 93.6200
803 CA SER 70 0.0018 90.0634 90.8900
814 CA VAL 71 0.0022 90.4372 91.0300
830 CA ALA 72 0.0019 90.1135 91.6600
840 CA ASP 73 0.0018 89.9878 95.1600
852 CA LEU 74 0.0021 90.3996 91.1400
871 CA ILE 75 0.0022 90.5256 91.5900
890 CA VAL 76 0.0019 90.1590 94.8000
906 CA GLY 77 0.0020 90.2837 92.8600
913 CA ALA 78 0.0025 90.8195 92.9600
923 CA VAL 79 0.0024 90.7489 96.5200
939 CA VAL 80 0.0021 90.3910 96.0100
955 CA MET 81 0.0019 90.1836 97.1900
972 CA PRO 82 0.0028 91.1146 96.0000
986 CA MET 83 0.0026 90.9399 96.2600
1003 CA ASN 84 0.0024 90.7513 96.1700
1017 CA ILE 85 0.0027 91.0051 91.2900
1036 CA LEU 86 0.0042 92.7238 94.1000
1055 CA TYR 87 0.0035 91.9967 97.9700
1076 CA LEU 88 0.0040 92.5168 94.3000
1095 CA LEU 89 0.0053 94.0154 89.6700
1114 CA MET 90 0.0071 96.0909 94.0400
1131 CA SER 91 0.0083 97.4106 95.9600
1142 CA LYS 92 0.0073 96.2521 97.6000
1164 CA TRP 93 0.0052 93.9442 99.5400
1188 CA SER 94 0.0092 98.4844 98.1300
1199 CA LEU 95 0.0083 97.3866 96.3100
1218 CA GLY 96 0.0097 99.0328 96.0500
1225 CA ARG 97 0.0071 96.0527 95.9000
1249 CA PRO 98 0.0100 99.3468 93.8600
1263 CA LEU 99 0.0064 95.2893 94.9000
1282 CA CYS 100 0.0036 92.0410 97.3100
1292 CA LEU 101 0.0036 92.0913 94.5500
1311 CA PHE 102 0.0036 92.0481 95.2800
1331 CA TRP 103 0.0026 90.9323 97.3400
1355 CA LEU 104 0.0024 90.6723 96.6400
1374 CA SER 105 0.0027 91.0057 95.5600
1385 CA MET 106 0.0023 90.5981 96.3000
1402 CA ASP 107 0.0020 90.2919 98.7900
1414 CA TYR 108 0.0020 90.2126 97.5500
1435 CA VAL 109 0.0023 90.5624 94.9800
1451 CA ALA 110 0.0019 90.1025 94.7300
1461 CA SER 111 0.0019 90.0841 97.9200
1472 CA THR 112 0.0022 90.4708 97.4800
1486 CA ALA 113 0.0020 90.2898 95.6100
1496 CA SER 114 0.0017 89.8711 98.0100
1507 CA ILE 115 0.0017 89.8940 99.5300
1526 CA PHE 116 0.0019 90.1187 98.5400
1546 CA SER 117 0.0017 89.8954 97.9800
1557 CA VAL 118 0.0016 89.8465 98.3000
1573 CA PHE 119 0.0018 90.0327 96.2000
1593 CA ILE 120 0.0018 90.0326 97.4000
1612 CA LEU 121 0.0014 89.6069 96.2400
1631 CA CYS 122 0.0017 89.9054 98.1100
1642 CA ILE 123 0.0022 90.4976 95.8400
1661 CA ASP 124 0.0023 90.5397 98.2200
1673 CA ARG 125 0.0018 90.0447 97.3300
1697 CA TYR 126 0.0030 91.3617 93.5700
1718 CA ARG 127 0.0041 92.5837 95.8800
1742 CA SER 128 0.0044 92.9924 97.9500
1753 CA VAL 129 0.0043 92.8755 98.5100
1769 CA GLN 130 0.0092 98.3965 99.7600
1786 CA GLN 131 0.0128 102.5101 100.9600
1803 CA PRO 132 0.0138 103.7001 101.8300
1817 CA LEU 133 0.0216 112.5558 98.3200
1836 CA ARG 134 0.0172 107.5424 96.3600
1860 CA TYR 135 0.0093 98.5869 95.5700
1881 CA LEU 136 0.0131 102.8149 94.7600
1900 CA LYS 137 0.0194 110.0282 93.2300
1922 CA TYR 138 0.0100 99.2976 95.3900
1943 CA ARG 139 0.0066 95.5011 98.6700
1967 CA THR 140 0.0092 98.4209 97.6600
1981 CA LYS 141 0.0067 95.6304 97.8700
2003 CA THR 142 0.0114 100.9753 95.9200
2017 CA ARG 143 0.0086 97.7077 101.3900
2041 CA ALA 144 0.0043 92.8786 99.5100
2051 CA SER 145 0.0060 94.8200 100.3600
2062 CA ALA 146 0.0078 96.8606 100.4300
2072 CA THR 147 0.0056 94.2966 99.7700
2086 CA ILE 148 0.0033 91.7858 97.8900
2105 CA LEU 149 0.0068 95.7124 98.1300
2124 CA GLY 150 0.0062 95.0677 98.4200
2131 CA ALA 151 0.0036 92.0833 98.5100
2141 CA TRP 152 0.0035 91.9275 98.5400
2165 CA PHE 153 0.0061 94.9357 98.7000
2185 CA LEU 154 0.0052 93.9277 96.9200
2204 CA SER 155 0.0033 91.7094 95.8200
2215 CA PHE 156 0.0039 92.4393 95.7200
2235 CA LEU 157 0.0057 94.4456 97.3800
2254 CA TRP 158 0.0030 91.4324 94.9200
2278 CA VAL 159 0.0036 92.0489 93.2100
2294 CA ILE 160 0.0061 94.8695 93.6600
2313 CA PRO 161 0.0041 92.6783 91.6800
2327 CA ILE 162 0.0029 91.2852 93.3500
2346 CA LEU 163 0.0042 92.7301 91.6700
2365 CA GLY 164 0.0072 96.1176 86.1900
2372 CA TRP 165 0.0056 94.3736 89.1200
2396 CA ASN 166 0.0110 100.4301 83.8600
2410 CA HIS 167 0.0188 109.3638 80.6300
2427 CA PHE 168 0.0145 104.4129 81.5400
2447 CA MET 169 0.0142 104.1383 84.5200
2464 CA GLN 170 0.0306 122.7128 88.7900
2481 CA GLN 171 0.0214 112.2856 91.4400
2498 CA VAL 174 0.0090 98.2226 114.3100
2514 CA ARG 175 0.0204 111.1943 120.7300
2538 CA ARG 176 0.0126 102.3134 119.5500
2562 CA GLU 177 0.0113 100.8463 117.5100
2577 CA ASP 178 0.0122 101.8021 117.5400
2589 CA LYS 179 0.0075 96.4947 114.4800
2611 CA CYS 180 0.0040 92.5711 111.5200
2621 CA GLU 181 0.0041 92.6008 108.3300
2636 CA THR 182 0.0028 91.1853 102.4500
2650 CA ASP 183 0.0033 91.7312 97.3100
2662 CA PHE 184 0.0032 91.6329 98.2000
2682 CA TYR 185 0.0037 92.1385 102.8800
2703 CA ASP 186 0.0064 95.2395 101.6500
2715 CA VAL 187 0.0055 94.1890 99.6800
2731 CA THR 188 0.0065 95.3245 98.6000
2745 CA TRP 189 0.0074 96.3942 97.4300
2769 CA PHE 190 0.0047 93.2871 96.4000
2789 CA LYS 191 0.0037 92.1413 98.4400
2811 CA VAL 192 0.0043 92.9033 96.8000
2827 CA MET 193 0.0057 94.4853 94.9000
2844 CA THR 194 0.0039 92.3637 94.6900
2858 CA ALA 195 0.0032 91.6199 95.2800
2868 CA ILE 196 0.0042 92.7894 94.9300
2887 CA ILE 197 0.0054 94.1006 94.6900
2906 CA ASN 198 0.0030 91.3650 98.0100
2920 CA PHE 199 0.0025 90.8088 99.5900
2940 CA TYR 200 0.0036 92.0736 99.6300
2961 CA LEU 201 0.0046 93.2382 94.8200
2980 CA PRO 202 0.0029 91.3118 96.3100
2994 CA THR 203 0.0028 91.2042 97.8800
3008 CA LEU 204 0.0048 93.4255 95.7100
3027 CA LEU 205 0.0042 92.7129 94.9000
3046 CA MET 206 0.0024 90.7583 97.7400
3063 CA LEU 207 0.0033 91.7153 94.9700
3082 CA TRP 208 0.0052 93.8404 93.7500
3106 CA PHE 209 0.0032 91.6219 93.2200
3126 CA TYR 210 0.0029 91.3020 95.4000
3147 CA ALA 211 0.0045 93.1310 93.5000
3157 CA LYS 212 0.0051 93.7272 90.6100
3179 CA ILE 213 0.0036 92.1214 90.6500
3198 CA TYR 214 0.0066 95.4810 87.4200
3219 CA LYS 215 0.0107 100.0862 81.3700
3241 CA ALA 216 0.0087 97.8974 82.4900
3251 CA VAL 217 0.0072 96.1908 83.3100
3267 CA LYS 218 0.0144 104.3454 76.7400
3289 CA ARG 219 0.0182 108.6314 76.8700
3313 CA GLN 220 0.0178 108.2042 76.3600
3330 CA LEU 221 0.0223 113.2657 79.2800
3349 CA ASN 408 0.0185 109.0229 83.2600
3363 CA ARG 409 0.0134 103.2273 85.2800
3387 CA GLU 410 0.0075 96.4893 86.0200
3402 CA ARG 411 0.0083 97.4324 84.0700
3426 CA LYS 412 0.0064 95.2900 85.8900
3448 CA ALA 413 0.0043 92.8606 92.0800
3458 CA ALA 414 0.0034 91.8843 94.0000
3468 CA LYS 415 0.0042 92.7894 93.4800
3490 CA GLN 416 0.0020 90.2920 95.3900
3507 CA LEU 417 0.0017 89.9087 98.3600
3526 CA GLY 418 0.0028 91.1676 98.3000
3533 CA PHE 419 0.0029 91.2704 103.7700
3553 CA ILE 420 0.0019 90.1140 101.1600
3572 CA MET 421 0.0020 90.2625 102.2800
3589 CA ALA 422 0.0027 91.1044 102.1900
3599 CA ALA 423 0.0025 90.7666 103.2600
3609 CA PHE 424 0.0020 90.2681 104.4600
3629 CA ILE 425 0.0025 90.7734 101.3300
3648 CA LEU 426 0.0044 92.9606 104.4900
3667 CA CYS 427 0.0030 91.3384 105.9300
3678 CA TRP 428 0.0023 90.5828 103.1400
3702 CA ILE 429 0.0028 91.1960 102.9300
3721 CA PRO 430 0.0033 91.7415 102.8600
3735 CA TYR 431 0.0027 91.0789 101.5900
3756 CA PHE 432 0.0028 91.1755 100.0100
3776 CA ILE 433 0.0038 92.2781 102.7100
3795 CA PHE 434 0.0039 92.4331 104.4200
3815 CA PHE 435 0.0031 91.5009 102.1100
3835 CA MET 436 0.0041 92.5991 103.1700
3852 CA VAL 437 0.0062 95.0697 104.2900
3868 CA ILE 438 0.0056 94.3478 105.6300
3887 CA ALA 439 0.0055 94.1949 105.5200
3897 CA PHE 440 0.0117 101.3196 108.2700
3917 CA CYS 441 0.0144 104.3923 118.1400
3927 CA LYS 442 0.0223 113.3604 123.7600
3949 CA ASN 443 0.0431 136.9099 124.7900
3963 CA CYS 444 0.0199 110.6165 117.0000
3973 CA CYS 445 0.0185 108.9776 117.4400
3984 CA ASN 446 0.0142 104.0690 109.2600
3998 CA GLU 447 0.0139 103.7796 110.0600
4013 CA HIS 448 0.0140 103.9295 105.1700
4030 CA LEU 449 0.0081 97.1298 107.0800
4049 CA HIS 450 0.0043 92.8799 107.9800
4066 CA MET 451 0.0045 93.1270 105.9900
4083 CA PHE 452 0.0051 93.7968 105.2800
4103 CA THR 453 0.0036 92.0515 103.7500
4117 CA ILE 454 0.0027 91.0722 101.6000
4136 CA TRP 455 0.0024 90.7101 97.8300
4160 CA LEU 456 0.0029 91.2557 103.4400
4179 CA GLY 457 0.0024 90.7357 101.8700
4186 CA TYR 458 0.0020 90.2817 100.6500
4207 CA ILE 459 0.0024 90.7458 102.5900
4226 CA ASN 460 0.0021 90.3370 103.4700
4240 CA SER 461 0.0019 90.1630 99.8200
4251 CA THR 462 0.0023 90.5773 100.4400
4265 CA LEU 463 0.0024 90.7322 99.7200
4284 CA ASN 464 0.0019 90.0910 99.2800
4298 CA PRO 465 0.0020 90.2454 98.1300
4312 CA LEU 466 0.0026 90.9791 97.9500
4331 CA ILE 467 0.0025 90.7992 98.8400
4350 CA TYR 468 0.0022 90.5250 97.1300
4371 CA PRO 469 0.0033 91.7236 97.6800
4385 CA LEU 470 0.0045 93.1266 97.3800
4404 CA CYS 471 0.0037 92.1922 96.5400
4415 CA ASN 472 0.0046 93.1848 98.5200
4429 CA GLU 473 0.0087 97.8518 99.6100
4444 CA ASN 474 0.0059 94.6360 97.0200
4458 CA PHE 475 0.0039 92.3885 93.8200
4478 CA LYS 476 0.0075 96.5080 93.9000
4500 CA LYS 477 0.0098 99.1594 91.9200
4522 CA THR 478 0.0068 95.7089 88.7900
4536 CA PHE 479 0.0067 95.6122 87.4000
4556 CA LYS 480 0.0213 112.1462 85.5400
4578 CA ARG 481 0.0326 125.0572 83.2700

If you find results from this site helpful for your research, please cite one of our papers:

K. Suhre & Y.H. Sanejouand, ElNemo: a normal mode web-server for protein movement analysis and the generation of templates for molecular replacement. Nucleic Acids Research, 32, W610-W614, 2004.
K. Suhre & Y.H. Sanejouand, On the potential of normal mode analysis for solving difficult molecular replacement problems. Acta Cryst. D vol.60, p796-799, 2004 © International Union of Crystallography.

elNémo is maintained by Yves-Henri Sanejouand.
It was developed by Karsten Suhre.
Between 2003 and 2014, it was hosted by IGS (Marseille).
Between 2015 and 2025, it was hosted by US2B (Nantes).
Last modification: december 26th, 2025.

Should you encounter any unexpected behaviour, please let us know. elNémo has been hacked on november 27th. It has been moved away and runs again. **Still some additional cleaning from time to time** Sorry for the inconvenience.