Should you encounter any unexpected behaviour,
please let us know.
elNémo has been hacked on november 27th.
It has been moved away and runs again.
Still some additional cleaning from time to time
Sorry for the inconvenience.

* TRANSFERASE 18-APR-07 2PL0 *

B-factor analysis for ID 260201014444812257

Overall B-factor prediction

Correlation= 0.556 for 268 C-alpha atoms.

B-factors for all residues

Residue-by-residue B-factor prediction for CA atoms

B-factors are computed based on the first 100 normal modes (B-PREDICT) and are scaled to match the overall B-factors in the submitted model (B-SCALED). A high correlation with the observations is an indicator that the normal modes capture well the overall flexibility features of the model. Comparison of the observed (B-OBS, blue) and the computed (B-SCALED, red) B-factors for all C-alpha atoms is presented below. Differences between B-factors from normal mode analysis and from X-ray crystallographic data may for example indicate regions where the protein flexibility is modified due to external factors such as tight crystall-packing.

ATOM TYP RES RESID B-PREDICT B-SCALED B-OBS	B-SCALED	B-OBS
2 CA LYS 231 0.3316 118.9181 94.7400
7 CA PRO 232 0.1797 85.4283 92.4800
14 CA TRP 233 0.0719 61.6729 89.4700
28 CA TRP 234 0.1002 67.9058 87.1700
42 CA GLU 235 0.1303 74.5414 84.7800
51 CA ASP 236 0.0693 61.0891 79.0900
59 CA GLU 237 0.0521 57.3004 73.2100
68 CA TRP 238 0.0199 50.2029 67.5600
82 CA GLU 239 0.0228 50.8374 63.8700
91 CA VAL 240 0.0220 50.6742 58.3600
98 CA PRO 241 0.0317 52.8030 57.7200
105 CA ARG 242 0.0264 51.6363 61.4300
116 CA GLU 243 0.0545 57.8378 62.5800
125 CA THR 244 0.0344 53.4026 60.3900
132 CA LEU 245 0.0225 50.7854 63.3400
140 CA LYS 246 0.0313 52.7159 68.5800
149 CA LEU 247 0.0289 52.1837 70.5200
157 CA VAL 248 0.0427 55.2279 73.9800
164 CA GLU 249 0.0523 57.3552 79.3800
173 CA ARG 250 0.0477 56.3345 82.9200
184 CA LEU 251 0.0515 57.1614 82.6800
192 CA GLY 252 0.0595 58.9312 84.5800
196 CA ALA 253 0.0446 55.6506 88.6600
201 CA GLY 254 0.0414 54.9338 88.3800
205 CA GLN 255 0.0425 55.1868 90.0500
214 CA PHE 256 0.0353 53.5960 83.7800
225 CA GLY 257 0.0315 52.7570 79.9100
229 CA GLU 258 0.0291 52.2353 78.0400
238 CA VAL 259 0.0262 51.5984 71.0100
245 CA TRP 260 0.0249 51.3133 69.1000
259 CA MET 261 0.0256 51.4709 63.8300
267 CA GLY 262 0.0240 51.1072 58.5900
271 CA TYR 263 0.0259 51.5243 58.0800
283 CA TYR 264 0.0236 51.0313 59.6700
295 CA ASN 265 0.0492 56.6531 64.7400
303 CA GLY 266 0.0594 58.9199 69.0800
307 CA HIS 267 0.0667 60.5183 70.5400
317 CA THR 268 0.0392 54.4553 66.0400
324 CA LYS 269 0.0236 51.0245 60.5700
333 CA VAL 270 0.0169 49.5484 52.2800
340 CA ALA 271 0.0157 49.2702 49.2300
345 CA VAL 272 0.0132 48.7381 51.6800
352 CA LYS 273 0.0149 49.0954 52.4200
361 CA SER 274 0.0162 49.4012 58.4800
367 CA LEU 275 0.0250 51.3326 65.1500
375 CA LYS 276 0.0360 53.7514 72.5600
384 CA GLN 277 0.0659 60.3374 78.1800
393 CA GLY 278 0.0861 64.7926 80.0200
397 CA SER 279 0.0612 59.3173 78.9100
403 CA MET 280 0.0596 58.9646 77.0200
411 CA SER 281 0.0745 62.2502 77.1800
417 CA PRO 282 0.0504 56.9393 77.7400
424 CA ASP 283 0.0794 63.3158 77.7300
432 CA ALA 284 0.0636 59.8297 68.4600
437 CA PHE 285 0.0287 52.1452 59.3800
448 CA LEU 286 0.0272 51.8204 54.4500
456 CA ALA 287 0.0425 55.1854 49.7100
461 CA GLU 288 0.0290 52.2092 47.8300
470 CA ALA 289 0.0200 50.2227 49.5300
475 CA ASN 290 0.0282 52.0265 50.2900
483 CA LEU 291 0.0284 52.0701 47.3000
491 CA MET 292 0.0183 49.8449 45.7600
499 CA LYS 293 0.0199 50.2041 45.6400
508 CA GLN 294 0.0275 51.8810 45.9100
517 CA LEU 295 0.0197 50.1582 48.1800
525 CA GLN 296 0.0167 49.5069 52.3400
534 CA HIS 297 0.0169 49.5527 47.4200
544 CA GLN 298 0.0175 49.6683 46.8000
553 CA ARG 299 0.0113 48.3064 44.3400
564 CA LEU 300 0.0127 48.6166 43.2400
572 CA VAL 301 0.0139 48.8811 44.3900
579 CA ARG 302 0.0125 48.5663 51.7200
590 CA LEU 303 0.0138 48.8518 50.7500
598 CA TYR 304 0.0120 48.4713 54.4100
610 CA ALA 305 0.0116 48.3866 54.0500
615 CA VAL 306 0.0115 48.3581 56.0900
622 CA VAL 307 0.0166 49.4753 61.5900
629 CA THR 308 0.0272 51.8067 70.5000
636 CA GLN 309 0.0529 57.4755 76.3900
645 CA GLU 310 0.0410 54.8506 76.3600
654 CA PRO 311 0.0314 52.7510 71.6700
661 CA ILE 312 0.0204 50.3125 62.6900
669 CA TYR 313 0.0119 48.4356 55.0200
681 CA ILE 314 0.0108 48.2039 47.2200
689 CA ILE 315 0.0087 47.7299 48.2000
697 CA THR 316 0.0114 48.3318 49.7900
704 CA GLU 317 0.0168 49.5205 55.4200
713 CA TYR 318 0.0197 50.1623 56.0200
725 CA MET 319 0.0223 50.7371 56.8400
733 CA GLU 320 0.0369 53.9536 56.8400
742 CA ASN 321 0.0219 50.6519 51.3100
750 CA GLY 322 0.0217 50.6049 45.3300
754 CA SER 323 0.0156 49.2616 41.6300
760 CA LEU 324 0.0114 48.3322 39.3100
768 CA VAL 325 0.0161 49.3596 41.9000
775 CA ASP 326 0.0236 51.0299 42.6900
783 CA PHE 327 0.0208 50.4039 40.3500
794 CA LEU 328 0.0179 49.7610 43.0600
802 CA LYS 329 0.0320 52.8791 43.5700
811 CA THR 330 0.0414 54.9395 44.9000
818 CA PRO 331 0.0804 63.5384 50.1900
825 CA SER 332 0.0591 58.8564 54.5500
831 CA GLY 333 0.0309 52.6234 53.7400
835 CA ILE 334 0.0384 54.2857 50.5600
843 CA LYS 335 0.0638 59.8862 51.3500
852 CA LEU 336 0.0395 54.5359 48.1400
860 CA THR 337 0.0465 56.0580 46.4300
867 CA ILE 338 0.0295 52.3272 44.9200
875 CA ASN 339 0.0304 52.5257 46.3400
883 CA LYS 340 0.0222 50.7225 47.8900
892 CA LEU 341 0.0141 48.9295 42.6900
900 CA LEU 342 0.0123 48.5367 39.8800
908 CA ASP 343 0.0134 48.7700 42.0700
916 CA MET 344 0.0107 48.1878 37.8300
924 CA ALA 345 0.0085 47.6936 34.9400
929 CA ALA 346 0.0092 47.8501 37.0400
934 CA GLN 347 0.0094 47.8840 36.4400
943 CA ILE 348 0.0076 47.4874 34.4600
951 CA ALA 349 0.0083 47.6581 33.8200
956 CA GLU 350 0.0100 48.0328 39.0200
965 CA GLY 351 0.0104 48.1068 37.4100
969 CA MET 352 0.0094 47.8835 35.9800
977 CA ALA 353 0.0145 49.0057 36.1900
982 CA PHE 354 0.0147 49.0529 37.5800
993 CA ILE 355 0.0149 49.1026 40.0700
1001 CA GLU 356 0.0153 49.1899 44.6500
1010 CA GLU 357 0.0334 53.1885 47.1700
1019 CA ARG 358 0.0294 52.3020 46.6400
1030 CA ASN 359 0.0361 53.7735 43.7400
1038 CA TYR 360 0.0287 52.1538 38.8600
1050 CA ILE 361 0.0163 49.4063 38.4300
1058 CA HIS 362 0.0132 48.7347 40.2300
1068 CA ARG 363 0.0135 48.8038 44.8500
1079 CA ASP 364 0.0108 48.1953 41.6600
1087 CA LEU 365 0.0076 47.4896 39.9000
1095 CA ARG 366 0.0073 47.4349 37.8400
1106 CA ALA 367 0.0088 47.7561 33.4300
1111 CA ALA 368 0.0113 48.3029 35.4000
1116 CA ASN 369 0.0104 48.1198 38.6000
1124 CA ILE 370 0.0095 47.9114 38.2800
1132 CA LEU 371 0.0120 48.4626 40.6000
1140 CA VAL 372 0.0133 48.7429 40.4500
1147 CA SER 373 0.0231 50.9055 46.8600
1153 CA ASP 374 0.0439 55.5026 55.7500
1161 CA THR 375 0.0499 56.8220 51.2200
1168 CA LEU 376 0.0229 50.8608 49.4100
1176 CA SER 377 0.0157 49.2798 47.5100
1182 CA CYS 378 0.0120 48.4689 44.4600
1188 CA LYS 379 0.0101 48.0462 43.5300
1197 CA ILE 380 0.0098 47.9825 38.9800
1205 CA ALA 381 0.0131 48.7112 37.8400
1210 CA ASP 382 0.0151 49.1440 41.3400
1218 CA PHE 383 0.0200 50.2349 41.0800
1229 CA GLY 384 0.0285 52.0905 43.9300
1233 CA LEU 385 0.0251 51.3481 47.7100
1241 CA ALA 386 0.0372 54.0124 58.4900
1246 CA ARG 387 0.0376 54.1001 71.4700
1257 CA LEU 388 0.0349 53.5113 79.5700
1265 CA ILE 389 0.0636 59.8353 88.3200
1273 CA GLU 390 0.0718 61.6436 94.2500
1282 CA ASP 391 0.1275 73.9334 92.2900
1290 CA ASN 392 0.2668 104.6185 82.8700
1298 CA GLU 393 0.6176 181.9477 79.2100
1307 CA TYR 394 0.6483 188.7165 78.9500
1319 CA THR 395 0.3004 112.0387 86.8500
1326 CA ALA 396 0.3209 116.5547 94.1700
1331 CA ARG 397 0.2184 93.9545 100.1100
1342 CA GLU 398 0.3777 129.0711 102.2500
1351 CA GLY 399 0.2533 101.6546 98.4000
1355 CA ALA 400 0.0950 66.7577 89.5500
1360 CA LYS 401 0.0342 53.3519 76.1100
1369 CA PHE 402 0.0242 51.1634 63.2200
1380 CA PRO 403 0.0169 49.5480 53.2400
1387 CA ILE 404 0.0176 49.7046 48.4200
1395 CA LYS 405 0.0126 48.5954 45.5900
1404 CA TRP 406 0.0088 47.7481 43.8000
1418 CA THR 407 0.0114 48.3417 44.1600
1425 CA ALA 408 0.0114 48.3296 44.8400
1430 CA PRO 409 0.0194 50.1062 47.7600
1437 CA GLU 410 0.0225 50.7802 46.2700
1446 CA ALA 411 0.0223 50.7452 49.8200
1451 CA ILE 412 0.0247 51.2699 53.3800
1459 CA ASN 413 0.0559 58.1507 55.7500
1467 CA TYR 414 0.0723 61.7599 56.9600
1479 CA GLY 415 0.0446 55.6420 53.5900
1483 CA THR 416 0.0399 54.6206 54.0400
1490 CA PHE 417 0.0207 50.3784 51.7800
1501 CA THR 418 0.0187 49.9480 47.0400
1508 CA ILE 419 0.0131 48.6992 42.4800
1516 CA LYS 420 0.0110 48.2330 42.4400
1525 CA SER 421 0.0103 48.0874 41.3000
1531 CA ASP 422 0.0084 47.6667 41.7700
1539 CA VAL 423 0.0069 47.3338 39.2700
1546 CA TRP 424 0.0061 47.1582 38.6300
1560 CA SER 425 0.0060 47.1452 38.6500
1566 CA PHE 426 0.0046 46.8303 33.4300
1577 CA GLY 427 0.0050 46.9263 33.7600
1581 CA ILE 428 0.0056 47.0548 34.3800
1589 CA LEU 429 0.0057 47.0719 37.1800
1597 CA LEU 430 0.0054 47.0119 35.1000
1605 CA THR 431 0.0079 47.5526 38.4500
1612 CA GLU 432 0.0090 47.7924 38.8100
1621 CA ILE 433 0.0092 47.8558 42.4000
1629 CA VAL 434 0.0117 48.3967 44.1200
1636 CA THR 435 0.0182 49.8415 45.4500
1643 CA HIS 436 0.0196 50.1363 48.1800
1653 CA GLY 437 0.0201 50.2440 47.2000
1657 CA ARG 438 0.0220 50.6699 48.6100
1668 CA ILE 439 0.0235 50.9909 47.3800
1676 CA PRO 440 0.0149 49.1024 47.8000
1683 CA TYR 441 0.0214 50.5466 50.8300
1695 CA PRO 442 0.0344 53.3964 57.9900
1702 CA GLY 443 0.0735 62.0289 61.9500
1706 CA MET 444 0.0478 56.3485 61.3000
1714 CA THR 445 0.0564 58.2444 57.3200
1721 CA ASN 446 0.0429 55.2742 56.7400
1729 CA PRO 447 0.0619 59.4693 57.5200
1736 CA GLU 448 0.0517 57.2200 58.3700
1745 CA VAL 449 0.0286 52.1296 54.9400
1752 CA ILE 450 0.0328 53.0450 55.7600
1760 CA GLN 451 0.0515 57.1729 58.5500
1769 CA ASN 452 0.0390 54.4191 55.2200
1777 CA LEU 453 0.0270 51.7623 53.5600
1785 CA GLU 454 0.0490 56.6284 59.9900
1794 CA ARG 455 0.0643 59.9951 61.0700
1805 CA GLY 456 0.0502 56.8880 52.9900
1809 CA TYR 457 0.0256 51.4687 46.8500
1821 CA ARG 458 0.0184 49.8849 43.8100
1832 CA MET 459 0.0162 49.3934 44.7900
1840 CA VAL 460 0.0229 50.8637 41.4300
1847 CA ARG 461 0.0232 50.9251 40.3400
1858 CA PRO 462 0.0263 51.6131 44.1700
1865 CA ASP 463 0.0658 60.3229 52.3800
1873 CA ASN 464 0.0731 61.9371 60.9300
1881 CA CYS 465 0.0269 51.7384 52.3100
1887 CA PRO 466 0.0287 52.1457 46.8200
1894 CA GLU 467 0.0343 53.3771 42.0600
1903 CA GLU 468 0.0329 53.0741 43.4300
1912 CA LEU 469 0.0146 49.0408 36.0200
1920 CA TYR 470 0.0133 48.7516 35.8100
1932 CA GLN 471 0.0171 49.5788 39.5100
1941 CA LEU 472 0.0117 48.4064 41.0300
1949 CA MET 473 0.0083 47.6587 37.0900
1957 CA ARG 474 0.0112 48.2824 36.4200
1968 CA LEU 475 0.0107 48.1757 38.4800
1976 CA CYS 476 0.0073 47.4339 40.4600
1982 CA TRP 477 0.0086 47.7148 43.9400
1996 CA LYS 478 0.0135 48.7920 53.2300
2005 CA GLU 479 0.0216 50.5805 55.9500
2014 CA ARG 480 0.0299 52.4095 57.3100
2025 CA PRO 481 0.0194 50.0987 55.5800
2032 CA GLU 482 0.0379 54.1676 58.1300
2041 CA ASP 483 0.0277 51.9203 55.4600
2049 CA ARG 484 0.0154 49.2057 45.6000
2060 CA PRO 485 0.0114 48.3229 41.7900
2067 CA THR 486 0.0123 48.5384 44.5700
2074 CA PHE 487 0.0091 47.8297 42.7700
2085 CA ASP 488 0.0145 49.0225 47.1600
2093 CA TYR 489 0.0168 49.5298 45.9100
2105 CA LEU 490 0.0098 47.9720 44.2700
2113 CA ARG 491 0.0125 48.5654 46.2800
2124 CA SER 492 0.0181 49.8151 48.7400
2130 CA VAL 493 0.0186 49.9240 45.7900
2137 CA LEU 494 0.0133 48.7601 51.2800
2145 CA GLU 495 0.0247 51.2565 61.5800
2154 CA ASP 496 0.0444 55.6036 69.7800
2162 CA PHE 497 0.0339 53.2823 72.1700
2173 CA PHE 498 0.0553 58.0071 76.1200

If you find results from this site helpful for your research, please cite one of our papers:

K. Suhre & Y.H. Sanejouand, ElNemo: a normal mode web-server for protein movement analysis and the generation of templates for molecular replacement. Nucleic Acids Research, 32, W610-W614, 2004.
K. Suhre & Y.H. Sanejouand, On the potential of normal mode analysis for solving difficult molecular replacement problems. Acta Cryst. D vol.60, p796-799, 2004 © International Union of Crystallography.

elNémo is maintained by Yves-Henri Sanejouand.
It was developed by Karsten Suhre.
Between 2003 and 2014, it was hosted by IGS (Marseille).
Between 2015 and 2025, it was hosted by US2B (Nantes).
Last modification: december 26th, 2025.

Should you encounter any unexpected behaviour, please let us know. elNémo has been hacked on november 27th. It has been moved away and runs again. **Still some additional cleaning from time to time** Sorry for the inconvenience.