Should you encounter any unexpected behaviour,
please let us know.
elNémo has been hacked on november 27th.
It has been moved away and runs again.
Still some additional cleaning from time to time
Sorry for the inconvenience.

* SIGNALING PROTEIN 20-OCT-09 3KB3 *

B-factor analysis for ID 2602031626281395717

Overall B-factor prediction

Correlation= 0.478 for 294 C-alpha atoms.

B-factors for all residues

Residue-by-residue B-factor prediction for CA atoms

B-factors are computed based on the first 100 normal modes (B-PREDICT) and are scaled to match the overall B-factors in the submitted model (B-SCALED). A high correlation with the observations is an indicator that the normal modes capture well the overall flexibility features of the model. Comparison of the observed (B-OBS, blue) and the computed (B-SCALED, red) B-factors for all C-alpha atoms is presented below. Differences between B-factors from normal mode analysis and from X-ray crystallographic data may for example indicate regions where the protein flexibility is modified due to external factors such as tight crystall-packing.

ATOM TYP RES RESID B-PREDICT B-SCALED B-OBS	B-SCALED	B-OBS
2 CA CYS 186 0.0173 50.1324 86.0300
8 CA ILE 187 0.0141 48.2507 75.4900
16 CA PRO 188 0.0099 45.7639 60.2000
23 CA LEU 189 0.0081 44.6804 47.9500
31 CA TRP 190 0.0069 44.0083 42.5100
45 CA GLY 191 0.0079 44.5949 37.3500
49 CA THR 192 0.0075 44.3358 36.0400
56 CA VAL 193 0.0086 44.9708 33.0200
63 CA SER 194 0.0101 45.8657 31.9600
69 CA ILE 195 0.0133 47.7472 33.1900
77 CA GLN 196 0.0248 54.5754 35.3500
86 CA GLY 197 0.0282 56.5817 38.4600
90 CA ASN 198 0.0424 64.9636 45.5400
98 CA ARG 199 0.0403 63.7203 44.0000
109 CA SER 200 0.0903 93.2179 44.6400
115 CA GLU 201 0.0449 66.4331 37.3400
124 CA MET 202 0.0179 50.4658 30.8100
132 CA GLU 203 0.0131 47.6386 28.5700
141 CA ASP 204 0.0075 44.3397 29.3300
149 CA ALA 205 0.0075 44.3662 30.6600
154 CA PHE 206 0.0067 43.8430 31.5900
165 CA ALA 207 0.0070 44.0449 35.0400
170 CA VAL 208 0.0078 44.4863 42.8800
177 CA SER 209 0.0089 45.1381 53.4900
183 CA PRO 210 0.0116 46.7690 64.1200
190 CA HIS 211 0.0146 48.5227 80.8800
200 CA PHE 212 0.0126 47.3577 91.8600
211 CA LEU 213 0.0149 48.7107 108.2600
219 CA LYS 214 0.0334 59.6233 126.1700
228 CA LEU 215 0.0237 53.9212 138.3800
236 CA PRO 216 0.0452 66.6036 155.6200
243 CA ILE 217 0.0486 68.6066 162.3300
251 CA LYS 218 0.0470 67.6526 167.8000
260 CA MET 219 0.0243 54.2707 168.7600
268 CA LEU 220 0.0417 64.5094 169.9300
276 CA LEU 234 0.0252 54.7989 94.1700
284 CA THR 235 0.0191 51.1590 84.6400
291 CA GLY 236 0.0112 46.5084 70.3800
295 CA HIS 237 0.0091 45.2860 56.1200
305 CA PHE 238 0.0056 43.2334 44.3600
316 CA PHE 239 0.0055 43.1278 36.8400
327 CA GLY 240 0.0054 43.0878 30.4800
331 CA VAL 241 0.0048 42.7432 27.1700
338 CA TYR 242 0.0053 43.0303 23.7800
350 CA ASP 243 0.0057 43.2879 26.7000
358 CA GLY 244 0.0079 44.5820 25.5800
362 CA HIS 245 0.0103 46.0099 25.3300
372 CA GLY 246 0.0213 52.4721 24.4600
376 CA GLY 247 0.0174 50.1879 25.3400
380 CA HIS 248 0.0135 47.9019 28.4100
390 CA LYS 249 0.0133 47.7574 31.1500
399 CA VAL 250 0.0069 43.9714 28.3800
406 CA ALA 251 0.0073 44.2376 28.9100
411 CA ASP 252 0.0108 46.2704 32.1200
419 CA TYR 253 0.0085 44.9298 32.8200
431 CA CYS 254 0.0068 43.9325 34.5300
437 CA ARG 255 0.0087 45.0201 38.7800
448 CA ASP 256 0.0131 47.6343 41.8600
456 CA ARG 257 0.0099 45.7597 44.1900
467 CA LEU 258 0.0067 43.8817 44.0300
475 CA HIS 259 0.0078 44.5009 48.9100
485 CA PHE 260 0.0129 47.5156 55.7200
496 CA ALA 261 0.0117 46.8083 61.7200
501 CA LEU 262 0.0096 45.5575 68.3900
509 CA ALA 263 0.0136 47.9395 79.9300
514 CA GLU 264 0.0214 52.5367 91.8100
523 CA GLU 265 0.0196 51.4941 106.1000
532 CA ILE 266 0.0300 57.6244 121.1000
540 CA GLU 267 0.0867 91.1328 138.4300
549 CA ARG 268 0.2998 216.9930 148.8000
560 CA ARG 282 0.0539 71.7402 146.0000
571 CA GLN 283 0.0319 58.7681 134.7000
580 CA VAL 284 0.0413 64.2787 123.2000
587 CA GLN 285 0.0294 57.2915 109.6900
596 CA TRP 286 0.0113 46.5571 91.9400
610 CA ASP 287 0.0138 48.0563 85.6600
618 CA LYS 288 0.0267 55.6929 80.3300
627 CA VAL 289 0.0158 49.2550 69.6700
634 CA PHE 290 0.0076 44.3918 61.1900
645 CA THR 291 0.0094 45.4728 60.0600
652 CA SER 292 0.0122 47.1245 58.5300
658 CA CYS 293 0.0091 45.2935 51.7300
664 CA PHE 294 0.0062 43.5671 45.5800
675 CA LEU 295 0.0084 44.8773 46.9500
683 CA THR 296 0.0103 45.9651 46.8700
690 CA VAL 297 0.0083 44.8294 41.5200
697 CA ASP 298 0.0091 45.2747 40.2000
705 CA GLY 299 0.0124 47.2546 41.6900
709 CA GLU 300 0.0112 46.4993 43.9900
718 CA ILE 301 0.0104 46.0691 42.7600
726 CA GLU 302 0.0165 49.6660 50.0400
735 CA GLY 303 0.0231 53.5754 57.2500
739 CA LYS 304 0.0403 63.6898 63.3100
748 CA ILE 305 0.0314 58.4510 64.4500
756 CA GLY 306 0.0392 63.0467 66.8000
760 CA ARG 307 0.0343 60.1880 73.0300
771 CA ALA 308 0.0972 97.3411 84.3800
776 CA VAL 309 0.1484 127.5651 93.1400
783 CA VAL 310 0.5028 336.8941 100.4600
790 CA SER 312 0.4196 287.7151 135.4200
796 CA SER 313 0.3796 264.1104 135.3100
802 CA ASP 314 0.2002 158.1733 128.4500
810 CA LYS 315 0.0930 94.8208 115.2700
819 CA VAL 316 0.0654 78.5425 100.3100
826 CA LEU 317 0.0506 69.7978 84.8600
834 CA GLU 318 0.0405 63.8438 68.9500
843 CA ALA 319 0.0261 55.3196 52.8200
848 CA VAL 320 0.0161 49.4203 44.6400
855 CA ALA 321 0.0168 49.8043 36.1300
860 CA SER 322 0.0259 55.1765 32.0900
866 CA GLU 323 0.0212 52.4046 30.1700
875 CA THR 324 0.0192 51.2534 29.7500
882 CA VAL 325 0.0121 47.0315 28.0000
889 CA GLY 326 0.0070 44.0243 25.7500
893 CA SER 327 0.0062 43.5908 26.7600
899 CA THR 328 0.0048 42.7570 26.8900
906 CA ALA 329 0.0050 42.8317 29.4900
911 CA VAL 330 0.0045 42.5885 32.1500
918 CA VAL 331 0.0047 42.6998 36.6200
925 CA ALA 332 0.0055 43.1324 41.7300
930 CA LEU 333 0.0055 43.1813 49.4500
938 CA VAL 334 0.0075 44.3539 54.7300
945 CA CYS 335 0.0102 45.9565 61.0200
951 CA SER 336 0.0181 50.5728 67.7800
957 CA SER 337 0.0179 50.4934 69.8800
963 CA HIS 338 0.0117 46.8250 65.1200
973 CA ILE 339 0.0089 45.1556 56.5700
981 CA VAL 340 0.0070 44.0497 51.9000
988 CA VAL 341 0.0064 43.6714 45.9200
995 CA SER 342 0.0060 43.4772 42.1500
1001 CA ASN 343 0.0054 43.0814 38.9800
1009 CA CYS 344 0.0052 42.9845 36.4600
1015 CA GLY 345 0.0055 43.1413 33.0700
1019 CA ASP 346 0.0059 43.4205 32.2500
1027 CA SER 347 0.0057 43.2787 31.7200
1033 CA ARG 348 0.0063 43.6522 33.1900
1044 CA ALA 349 0.0060 43.4276 35.8700
1049 CA VAL 350 0.0076 44.3799 38.6300
1056 CA LEU 351 0.0072 44.1667 44.2300
1064 CA PHE 352 0.0103 45.9929 47.6400
1075 CA ARG 353 0.0129 47.5218 54.8500
1086 CA GLY 354 0.0325 59.0811 60.0600
1090 CA LYS 355 0.0321 58.8743 64.1900
1099 CA GLU 356 0.0263 55.4186 60.8500
1108 CA ALA 357 0.0157 49.1633 51.9400
1113 CA MET 358 0.0126 47.3232 50.4200
1121 CA PRO 359 0.0116 46.7698 45.3000
1128 CA LEU 360 0.0073 44.2302 41.8200
1136 CA SER 361 0.0078 44.4982 39.7700
1142 CA VAL 362 0.0104 46.0434 39.2800
1149 CA ASP 363 0.0086 44.9900 37.0800
1157 CA HIS 364 0.0085 44.9508 36.3000
1167 CA LYS 365 0.0106 46.1517 38.6400
1176 CA PRO 366 0.0157 49.1868 42.4500
1183 CA ASP 367 0.0289 56.9765 48.8600
1191 CA ARG 368 0.0323 58.9896 52.9900
1202 CA GLU 369 0.0549 72.3496 57.3500
1211 CA ASP 370 0.0392 63.0687 52.7400
1219 CA GLU 371 0.0196 51.4572 46.4400
1228 CA TYR 372 0.0290 57.0450 50.6900
1240 CA ALA 373 0.0410 64.1461 54.4800
1245 CA ARG 374 0.0294 57.2444 54.9500
1256 CA ILE 375 0.0219 52.8467 49.3800
1264 CA GLU 376 0.0441 65.9483 56.8500
1273 CA ASN 377 0.0724 82.6953 62.5800
1281 CA ALA 378 0.0526 70.9527 60.6000
1286 CA GLY 379 0.0571 73.6106 57.7300
1290 CA GLY 380 0.0356 60.9388 54.5800
1294 CA LYS 381 0.0352 60.7091 51.7500
1303 CA VAL 382 0.0306 57.9954 45.5100
1310 CA ILE 383 0.0452 66.5997 46.1500
1318 CA GLN 384 0.0808 87.6155 48.6100
1327 CA TRP 385 0.0843 89.6692 46.3100
1341 CA GLN 386 0.1239 113.1121 43.9800
1350 CA GLY 387 0.0929 94.7865 38.4600
1354 CA ALA 388 0.0370 61.7383 34.8000
1359 CA ARG 389 0.0263 55.4283 32.0300
1370 CA VAL 390 0.0179 50.4935 33.8100
1377 CA PHE 391 0.0303 57.8067 36.2700
1388 CA GLY 392 0.0408 64.0234 35.2000
1392 CA VAL 393 0.0373 61.9132 32.1500
1399 CA LEU 394 0.0165 49.6259 29.8200
1407 CA ALA 395 0.0173 50.1374 31.8900
1412 CA MET 396 0.0074 44.2983 33.3700
1420 CA SER 397 0.0071 44.0941 34.5800
1426 CA ARG 398 0.0055 43.1672 34.5400
1437 CA SER 399 0.0059 43.3740 35.0300
1443 CA ILE 400 0.0054 43.0956 33.4000
1451 CA GLY 401 0.0061 43.5175 33.4700
1455 CA ASP 402 0.0095 45.5045 32.6800
1463 CA ARG 403 0.0138 48.0728 36.0800
1474 CA TYR 404 0.0218 52.7878 35.4400
1486 CA LEU 405 0.0173 50.1503 33.1500
1494 CA LYS 406 0.0210 52.2955 43.9600
1503 CA PRO 407 0.0240 54.0833 47.5700
1510 CA TYR 408 0.0152 48.8754 46.8200
1522 CA VAL 409 0.0096 45.5626 45.2300
1529 CA ILE 410 0.0082 44.7538 44.6700
1537 CA PRO 411 0.0072 44.1669 43.4700
1544 CA GLU 412 0.0078 44.5393 47.7800
1553 CA PRO 413 0.0066 43.7926 47.6800
1560 CA GLU 414 0.0075 44.3172 51.2300
1569 CA VAL 415 0.0081 44.7005 54.1400
1576 CA THR 416 0.0112 46.5380 61.2400
1583 CA PHE 417 0.0112 46.5356 71.2500
1594 CA MET 418 0.0140 48.1493 76.3300
1602 CA PRO 419 0.0157 49.1927 77.5600
1609 CA ARG 420 0.0148 48.6662 73.5900
1620 CA SER 421 0.0190 51.1168 75.9100
1626 CA ARG 422 0.0436 65.6784 76.6200
1637 CA GLU 423 0.0285 56.7130 70.0500
1646 CA ASP 424 0.0111 46.4491 59.5700
1654 CA GLU 425 0.0096 45.5541 52.6900
1663 CA CYS 426 0.0055 43.1610 47.6900
1669 CA LEU 427 0.0046 42.6233 39.7300
1677 CA ILE 428 0.0039 42.2388 34.9500
1685 CA LEU 429 0.0044 42.4915 30.8000
1693 CA ALA 430 0.0053 43.0532 28.6400
1698 CA SER 431 0.0060 43.4661 27.8400
1704 CA ASP 432 0.0087 45.0527 28.8700
1712 CA GLY 433 0.0093 45.3803 28.2600
1716 CA LEU 434 0.0089 45.1444 29.6500
1724 CA TRP 435 0.0099 45.7622 33.5700
1738 CA ASP 436 0.0185 50.8517 37.8600
1746 CA VAL 437 0.0204 51.9749 39.5000
1753 CA MET 438 0.0161 49.4405 41.9100
1761 CA ASN 439 0.0185 50.8440 45.3900
1769 CA ASN 440 0.0138 48.0468 44.0800
1777 CA GLN 441 0.0162 49.4544 44.9700
1786 CA GLU 442 0.0164 49.6127 43.8500
1795 CA VAL 443 0.0109 46.3466 39.1700
1802 CA CYS 444 0.0096 45.5897 39.3700
1808 CA GLU 445 0.0128 47.4734 42.3900
1817 CA ILE 446 0.0118 46.8713 41.1800
1825 CA ALA 447 0.0088 45.1027 41.0900
1830 CA ARG 448 0.0106 46.1636 45.4900
1841 CA ARG 449 0.0138 48.0737 48.7000
1852 CA ARG 450 0.0124 47.2273 47.5500
1863 CA ILE 451 0.0104 46.0598 42.7700
1871 CA LEU 452 0.0190 51.1389 47.1600
1879 CA MET 453 0.0236 53.8428 55.1000
1887 CA TRP 454 0.0216 52.6704 62.5500
1901 CA HIS 455 0.0230 53.4837 76.5300
1911 CA LYS 456 0.0632 77.2435 86.7500
1920 CA LYS 457 0.0745 83.9138 94.1000
1929 CA ASN 458 0.0553 72.5402 100.7100
1937 CA GLY 459 0.0503 69.6236 108.5100
1941 CA ALA 460 0.0597 75.1877 115.2700
1946 CA PRO 461 0.0479 68.2080 119.7900
1953 CA PRO 462 0.1028 100.6352 123.8000
1960 CA LEU 463 0.0927 94.6602 124.6400
1968 CA ALA 464 0.1262 114.4297 122.0500
1973 CA GLU 465 0.0776 85.7404 116.5500
1982 CA ARG 466 0.0266 55.6073 106.8800
1993 CA GLY 467 0.0202 51.8322 96.2100
1997 CA LYS 468 0.0287 56.8819 86.9900
2006 CA GLY 469 0.0302 57.7218 72.3800
2010 CA ILE 470 0.0141 48.2367 61.6700
2018 CA ASP 471 0.0115 46.7125 55.6200
2026 CA PRO 472 0.0167 49.7964 52.6500
2033 CA ALA 473 0.0119 46.9232 46.4300
2038 CA CYS 474 0.0076 44.3773 42.4200
2044 CA GLN 475 0.0096 45.5799 41.3100
2053 CA ALA 476 0.0119 46.9200 40.7900
2058 CA ALA 477 0.0088 45.1289 34.8300
2063 CA ALA 478 0.0086 45.0093 36.1300
2068 CA ASP 479 0.0125 47.2645 41.2800
2076 CA TYR 480 0.0139 48.1159 38.7100
2088 CA LEU 481 0.0106 46.1582 35.3600
2096 CA SER 482 0.0121 47.0732 35.3700
2102 CA MET 483 0.0185 50.8387 43.0400
2110 CA LEU 484 0.0188 50.9818 40.8100
2118 CA ALA 485 0.0163 49.5259 38.0300
2123 CA LEU 486 0.0246 54.4518 44.2300
2131 CA GLN 487 0.0407 63.9601 51.5900
2140 CA LYS 488 0.0362 61.2707 51.7700
2149 CA GLY 489 0.0427 65.1227 50.2200
2153 CA SER 490 0.0243 54.2643 49.2400
2159 CA LYS 491 0.0338 59.8729 53.6500
2168 CA ASP 492 0.0171 50.0214 45.7000
2176 CA ASN 493 0.0134 47.7925 31.1900
2184 CA ILE 494 0.0086 44.9742 28.7500
2192 CA SER 495 0.0070 44.0550 28.4500
2198 CA ILE 496 0.0056 43.1873 27.9400
2206 CA ILE 497 0.0048 42.7628 30.1300
2214 CA VAL 498 0.0045 42.5759 32.3600
2221 CA ILE 499 0.0042 42.4174 35.3500
2229 CA ASP 500 0.0065 43.7286 42.5300
2237 CA LEU 501 0.0072 44.1893 48.5900
2245 CA LYS 502 0.0144 48.3881 61.8800
2254 CA ALA 503 0.0248 54.5704 74.3100
2259 CA GLN 504 0.0452 66.5869 82.9500
2268 CA ARG 505 0.0319 58.7499 86.6500
2279 CA LYS 506 0.1050 101.8950 94.4100

If you find results from this site helpful for your research, please cite one of our papers:

K. Suhre & Y.H. Sanejouand, ElNemo: a normal mode web-server for protein movement analysis and the generation of templates for molecular replacement. Nucleic Acids Research, 32, W610-W614, 2004.
K. Suhre & Y.H. Sanejouand, On the potential of normal mode analysis for solving difficult molecular replacement problems. Acta Cryst. D vol.60, p796-799, 2004 © International Union of Crystallography.

elNémo is maintained by Yves-Henri Sanejouand.
It was developed by Karsten Suhre.
Between 2003 and 2014, it was hosted by IGS (Marseille).
Between 2015 and 2025, it was hosted by US2B (Nantes).
Last modification: december 26th, 2025.

Should you encounter any unexpected behaviour, please let us know. elNémo has been hacked on november 27th. It has been moved away and runs again. **Still some additional cleaning from time to time** Sorry for the inconvenience.