Should you encounter any unexpected behaviour,
please let us know.
elNémo has been hacked on november 27th.
It has been moved away and runs again.
Still some additional cleaning from time to time
Sorry for the inconvenience.

* HYDROLASE 10-FEB-15 4Y4D *

B-factor analysis for ID 2602120905571230321

Overall B-factor prediction

Correlation= 0.589 for 349 C-alpha atoms.

B-factors for all residues

Residue-by-residue B-factor prediction for CA atoms

B-factors are computed based on the first 100 normal modes (B-PREDICT) and are scaled to match the overall B-factors in the submitted model (B-SCALED). A high correlation with the observations is an indicator that the normal modes capture well the overall flexibility features of the model. Comparison of the observed (B-OBS, blue) and the computed (B-SCALED, red) B-factors for all C-alpha atoms is presented below. Differences between B-factors from normal mode analysis and from X-ray crystallographic data may for example indicate regions where the protein flexibility is modified due to external factors such as tight crystall-packing.

ATOM TYP RES RESID B-PREDICT B-SCALED B-OBS	B-SCALED	B-OBS
2 CA SER 1 0.0069 11.1063 12.2400
3 CA SER 1 0.0070 11.1234 11.9000
25 CA THR 2 0.0047 10.1687 10.0500
39 CA GLY 3 0.0028 9.3733 8.8100
46 CA SER 4 0.0029 9.4159 8.6100
57 CA ALA 5 0.0027 9.3415 8.9000
67 CA THR 6 0.0030 9.4848 8.0700
81 CA THR 7 0.0025 9.2602 7.8000
95 CA THR 8 0.0032 9.5667 8.9900
109 CA PRO 9 0.0032 9.5389 9.2200
123 CA ILE 10 0.0041 9.9315 9.8500
142 CA ASP 11 0.0092 12.0431 10.5700
154 CA SER 12 0.0117 13.0950 13.5000
165 CA LEU 13 0.0048 10.2253 12.4600
184 CA ASP 14 0.0030 9.4853 10.6300
196 CA ASP 15 0.0036 9.7148 11.1700
208 CA ALA 16 0.0031 9.4931 10.1500
218 CA TYR 17 0.0019 9.0150 8.4900
239 CA ILE 18 0.0022 9.1511 7.5200
258 CA THR 19 0.0020 9.0625 7.2500
272 CA PRO 20 0.0022 9.1534 7.6800
286 CA VAL 21 0.0020 9.0606 7.2600
302 CA GLN 22 0.0025 9.2707 7.2800
319 CA ILE 23 0.0025 9.2632 8.3100
338 CA GLY 24 0.0037 9.7652 10.2100
345 CA THR 25 0.0107 12.6692 11.4100
359 CA PRO 26 0.0164 15.0767 11.6100
373 CA ALA 27 0.0067 11.0174 10.7800
383 CA GLN 28 0.0050 10.3087 9.6100
400 CA THR 29 0.0033 9.5997 8.1800
414 CA LEU 30 0.0025 9.2648 8.3000
433 CA ASN 31 0.0020 9.0708 7.6800
447 CA LEU 32 0.0016 8.8991 7.3900
466 CA ASP 33 0.0014 8.8044 7.2400
478 CA PHE 34 0.0013 8.7515 7.9700
498 CA ASP 35 0.0013 8.7425 7.7800
510 CA THR 36 0.0012 8.6995 7.9800
524 CA GLY 37 0.0017 8.9236 8.8000
531 CA SER 38 0.0017 8.9111 8.5600
542 CA SER 39 0.0017 8.9400 8.0300
553 CA ASP 40 0.0016 8.8882 8.1100
565 CA LEU 41 0.0011 8.6842 8.2000
584 CA TRP 42 0.0012 8.7219 8.2300
608 CA VAL 43 0.0016 8.8701 8.2200
624 CA PHE 44 0.0023 9.1567 8.8900
644 CA SER 45 0.0032 9.5706 10.7200
655 CA SER 46 0.0051 10.3546 13.2600
666 CA GLU 47 0.0048 10.2381 12.7400
681 CA THR 48 0.0049 10.2539 12.0600
695 CA THR 49 0.0123 13.3493 17.0200
709 CA ALA 50 0.0181 15.7752 19.1200
719 CA SER 51 0.0203 16.6929 20.1300
730 CA GLU 52 0.0098 12.3194 17.4200
745 CA VAL 53 0.0076 11.3816 15.4100
761 CA ASP 54 0.0107 12.6921 16.8600
762 CA ASP 54 0.0107 12.6887 16.4300
782 CA GLY 55 0.0099 12.3579 15.1600
789 CA GLN 56 0.0058 10.6311 11.4500
806 CA THR 57 0.0044 10.0584 10.2800
820 CA ILE 58 0.0036 9.7204 11.0200
839 CA TYR 59 0.0032 9.5436 9.7600
860 CA THR 60 0.0045 10.1004 11.1000
874 CA PRO 61 0.0046 10.1231 12.4100
888 CA SER 62 0.0110 12.8185 13.9300
899 CA LYS 63 0.0122 13.3123 14.1300
912 CA SER 64 0.0077 11.4479 12.4100
923 CA THR 65 0.0151 14.5304 15.0200
937 CA THR 66 0.0101 12.4411 12.3600
951 CA ALA 67 0.0056 10.5336 11.1800
961 CA LYS 68 0.0081 11.5831 12.4600
971 CA LEU 69 0.0086 11.7859 11.8000
990 CA LEU 70 0.0068 11.0636 11.0000
1009 CA SER 71 0.0205 16.7615 11.1700
1020 CA GLY 72 0.0197 16.4529 11.3500
1027 CA ALA 73 0.0068 11.0417 11.8400
1037 CA THR 74 0.0065 10.9439 11.8900
1051 CA TRP 75 0.0040 9.8994 10.5300
1075 CA SER 76 0.0049 10.2499 12.8400
1086 CA ILE 77 0.0056 10.5438 12.6100
1105 CA SER 78 0.0088 11.8977 18.1800
1106 CA SER 78 0.0088 11.9052 18.7800
1124 CA TYR 79 0.0085 11.7492 17.3800
1145 CA GLY 80 0.0154 14.6317 23.3800
1152 CA ASP 81 0.0263 19.1853 25.0000
1164 CA GLY 82 0.0196 16.4136 21.6100
1171 CA SER 83 0.0090 11.9801 16.4500
1182 CA SER 84 0.0069 11.0779 14.2900
1193 CA SER 85 0.0044 10.0614 12.2900
1204 CA SER 86 0.0043 10.0151 11.0800
1215 CA GLY 87 0.0043 10.0187 10.4600
1222 CA ASP 88 0.0040 9.8927 11.8900
1234 CA VAL 89 0.0031 9.5151 9.9900
1250 CA TYR 90 0.0033 9.5840 11.0300
1271 CA THR 91 0.0035 9.6748 11.0300
1285 CA ASP 92 0.0033 9.6065 9.4400
1297 CA THR 93 0.0033 9.5983 9.6600
1311 CA VAL 94 0.0021 9.1074 8.6800
1327 CA SER 95 0.0023 9.1626 8.4200
1338 CA VAL 96 0.0020 9.0391 8.3200
1354 CA GLY 97 0.0030 9.4868 10.7700
1361 CA GLY 98 0.0040 9.8932 11.5300
1368 CA LEU 99 0.0033 9.6112 9.4000
1387 CA THR 100 0.0036 9.7112 11.1300
1401 CA VAL 101 0.0034 9.6430 11.5600
1417 CA THR 102 0.0062 10.7957 12.6900
1431 CA GLY 103 0.0042 9.9831 12.4200
1438 CA GLN 104 0.0024 9.2002 8.8700
1455 CA ALA 105 0.0020 9.0398 9.1900
1465 CA VAL 106 0.0015 8.8520 9.1100
1481 CA GLU 107 0.0018 8.9577 8.9800
1496 CA SER 108 0.0022 9.1165 10.2300
1507 CA ALA 109 0.0030 9.4854 10.6800
1517 CA LYS 110 0.0048 10.2323 13.0400
1530 CA LYS 111 0.0063 10.8616 14.2500
1548 CA VAL 112 0.0057 10.5808 13.2500
1564 CA SER 113 0.0090 11.9713 14.7300
1575 CA SER 114 0.0127 13.5265 16.8600
1586 CA SER 115 0.0113 12.9179 16.7100
1587 CA SER 115 0.0112 12.9056 16.6800
1605 CA PHE 116 0.0061 10.7675 13.3100
1625 CA THR 117 0.0077 11.4394 13.5500
1639 CA GLU 118 0.0122 13.3129 16.5400
1654 CA ASP 119 0.0079 11.5097 14.5900
1666 CA SER 120 0.0080 11.5402 12.7400
1677 CA THR 121 0.0046 10.1214 11.5500
1691 CA ILE 122 0.0031 9.4959 9.0300
1710 CA ASP 123 0.0022 9.1337 8.4000
1722 CA GLY 124 0.0018 8.9589 8.5000
1729 CA LEU 125 0.0013 8.7509 7.9100
1748 CA LEU 126 0.0010 8.6496 7.6100
1767 CA GLY 127 0.0011 8.6926 7.7900
1774 CA LEU 128 0.0011 8.6755 7.8100
1793 CA ALA 129 0.0019 8.9968 8.1600
1803 CA PHE 130 0.0021 9.1002 8.9400
1823 CA SER 131 0.0029 9.4392 10.5200
1834 CA THR 132 0.0051 10.3248 11.8700
1848 CA LEU 133 0.0035 9.6573 10.9100
1867 CA ASN 134 0.0028 9.3908 9.2700
1881 CA THR 135 0.0042 9.9751 10.3700
1895 CA VAL 136 0.0041 9.9231 10.1200
1911 CA SER 137 0.0110 12.7853 12.2200
1922 CA PRO 138 0.0189 16.0871 12.2700
1936 CA THR 139 0.0108 12.7044 10.8100
1950 CA GLN 140 0.0065 10.9478 10.8300
1967 CA GLN 141 0.0031 9.4999 10.2300
1984 CA LYS 142 0.0027 9.3483 11.2100
2000 CA THR 143 0.0016 8.8684 8.7800
2014 CA PHE 144 0.0013 8.7770 9.4400
2034 CA PHE 145 0.0017 8.9371 9.0900
2054 CA ASP 146 0.0022 9.1370 12.3300
2055 CA ASP 146 0.0022 9.1333 12.1700
2075 CA ASN 147 0.0027 9.3314 11.4800
2089 CA ALA 148 0.0027 9.3330 11.5300
2099 CA LYS 149 0.0036 9.7218 16.4600
2115 CA ALA 150 0.0087 11.8646 19.9400
2125 CA SER 151 0.0071 11.1804 17.4700
2126 CA SER 151 0.0071 11.1744 17.4300
2144 CA LEU 152 0.0045 10.0928 12.7900
2163 CA ASP 153 0.0066 10.9514 12.6300
2175 CA SER 154 0.0049 10.2467 12.0700
2186 CA PRO 155 0.0030 9.4612 11.8200
2200 CA VAL 156 0.0020 9.0520 9.4000
2216 CA PHE 157 0.0012 8.7354 8.1000
2236 CA THR 158 0.0013 8.7766 7.2500
2250 CA ALA 159 0.0013 8.7729 7.9400
2260 CA ASP 160 0.0015 8.8574 7.6100
2272 CA LEU 161 0.0017 8.9359 7.7500
2291 CA GLY 162 0.0021 9.0823 9.3300
2298 CA TYR 163 0.0031 9.4959 11.4600
2319 CA HIS 164 0.0043 9.9954 13.0500
2335 CA ALA 165 0.0066 10.9746 12.0000
2345 CA PRO 166 0.0051 10.3356 10.0500
2359 CA GLY 167 0.0028 9.3642 8.4600
2366 CA THR 168 0.0023 9.1719 7.7800
2380 CA TYR 169 0.0016 8.8652 7.0900
2401 CA ASN 170 0.0018 8.9785 7.6800
2415 CA PHE 171 0.0018 8.9847 8.8500
2435 CA GLY 172 0.0025 9.2575 9.4400
2442 CA PHE 173 0.0029 9.4160 10.3700
2462 CA ILE 174 0.0029 9.4190 10.9600
2481 CA ASP 175 0.0067 10.9995 12.0500
2493 CA THR 176 0.0093 12.1082 15.3300
2507 CA THR 177 0.0189 16.0948 15.8100
2521 CA ALA 178 0.0073 11.2571 12.1500
2531 CA TYR 179 0.0054 10.4888 11.0900
2552 CA THR 180 0.0076 11.3824 11.7000
2566 CA GLY 181 0.0179 15.6749 11.6400
2574 CA SER 182 0.0096 12.2099 10.4900
2575 CA SER 182 0.0096 12.2054 10.6400
2595 CA ILE 183 0.0030 9.4768 9.6700
2615 CA THR 184 0.0030 9.4869 8.7900
2629 CA TYR 185 0.0022 9.1360 8.3300
2650 CA THR 186 0.0024 9.2094 8.3400
2664 CA ALA 187 0.0034 9.6209 9.9900
2674 CA VAL 188 0.0028 9.3823 10.0500
2690 CA SER 189 0.0044 10.0356 10.7900
2701 CA THR 190 0.0043 9.9988 14.5400
2715 CA LYS 191 0.0111 12.8334 16.6000
2722 CA GLN 192 0.0068 11.0644 15.2100
2739 CA GLY 193 0.0032 9.5715 11.1800
2746 CA PHE 194 0.0023 9.1853 9.4600
2766 CA TRP 195 0.0016 8.8724 8.6000
2790 CA GLU 196 0.0020 9.0361 9.6200
2805 CA TRP 197 0.0021 9.1019 8.6700
2829 CA THR 198 0.0024 9.2228 9.9400
2843 CA SER 199 0.0026 9.2976 8.2500
2854 CA THR 200 0.0034 9.6534 9.2900
2868 CA GLY 201 0.0032 9.5587 9.2300
2875 CA TYR 202 0.0027 9.3461 8.5200
2896 CA ALA 203 0.0035 9.6941 8.9200
2906 CA VAL 204 0.0035 9.6706 10.1600
2922 CA GLY 205 0.0069 11.0983 11.3300
2929 CA SER 206 0.0241 18.2929 15.5200
2930 CA SER 206 0.0242 18.3236 15.1300
2948 CA GLY 207 0.0215 17.1924 14.7800
2955 CA THR 208 0.0211 17.0386 16.4400
2956 CA THR 208 0.0213 17.1117 15.8700
2980 CA PHE 209 0.0075 11.3522 12.0200
3000 CA LYS 210 0.0082 11.6251 11.3100
3022 CA SER 211 0.0083 11.6709 13.0400
3033 CA THR 212 0.0076 11.3982 12.1100
3047 CA SER 213 0.0051 10.3283 11.7400
3058 CA ILE 214 0.0032 9.5524 10.4800
3077 CA ASP 215 0.0030 9.4582 10.1000
3078 CA ASP 215 0.0030 9.4596 10.7000
3098 CA GLY 216 0.0022 9.1372 8.9800
3105 CA ILE 217 0.0016 8.8951 8.8700
3124 CA ALA 218 0.0014 8.7952 9.2900
3134 CA ASP 219 0.0013 8.7569 8.4600
3146 CA THR 220 0.0013 8.7401 8.0200
3160 CA GLY 221 0.0016 8.8907 8.1700
3167 CA THR 222 0.0017 8.9182 8.4400
3181 CA THR 223 0.0023 9.1560 8.2100
3195 CA LEU 224 0.0018 8.9703 7.4700
3214 CA LEU 225 0.0015 8.8561 7.1200
3233 CA TYR 226 0.0019 9.0146 6.9500
3254 CA LEU 227 0.0023 9.1910 8.0500
3273 CA PRO 228 0.0036 9.7221 8.5400
3287 CA ALA 229 0.0058 10.6514 7.9900
3297 CA THR 230 0.0083 11.6856 8.5400
3311 CA VAL 231 0.0031 9.5073 8.0600
3327 CA VAL 232 0.0027 9.3426 7.5400
3343 CA SER 233 0.0051 10.3442 7.9400
3354 CA ALA 234 0.0047 10.1881 8.7800
3364 CA TYR 235 0.0030 9.4831 7.5400
3385 CA TRP 236 0.0034 9.6174 7.9000
3409 CA ALA 237 0.0080 11.5730 8.2100
3419 CA GLN 238 0.0075 11.3469 9.2200
3436 CA VAL 239 0.0074 11.3100 9.3300
3452 CA SER 240 0.0165 15.0910 13.4800
3453 CA SER 240 0.0165 15.0937 13.6400
3471 CA GLY 241 0.0140 14.0391 13.0600
3478 CA ALA 242 0.0077 11.4180 9.7300
3488 CA LYS 243 0.0121 13.2799 12.8400
3504 CA SER 244 0.0120 13.2136 12.3000
3515 CA SER 245 0.0202 16.6341 16.3100
3526 CA SER 246 0.0427 26.0465 24.3100
3537 CA SER 247 0.0468 27.7441 22.0700
3548 CA VAL 248 0.0240 18.2439 17.5600
3564 CA GLY 249 0.0221 17.4457 16.7800
3571 CA GLY 250 0.0107 12.6938 11.4200
3578 CA TYR 251 0.0051 10.3415 7.4700
3599 CA VAL 252 0.0061 10.7522 8.4300
3615 CA PHE 253 0.0063 10.8474 8.1900
3635 CA PRO 254 0.0098 12.3129 9.3400
3649 CA CYS 255 0.0131 13.6686 10.9700
3659 CA SER 256 0.0265 19.2932 11.4100
3670 CA ALA 257 0.0115 13.0073 11.9400
3680 CA THR 258 0.0144 14.2067 14.8700
3694 CA LEU 259 0.0038 9.8109 10.0300
3713 CA PRO 260 0.0041 9.9291 8.6400
3727 CA SER 261 0.0025 9.2464 7.9900
3738 CA PHE 262 0.0017 8.9366 7.4700
3758 CA THR 263 0.0019 9.0227 8.5700
3772 CA PHE 264 0.0016 8.8793 8.6000
3792 CA GLY 265 0.0024 9.2219 8.9000
3799 CA VAL 266 0.0022 9.1356 8.9000
3815 CA GLY 267 0.0068 11.0631 11.5600
3822 CA SER 268 0.0140 14.0506 14.9500
3833 CA ALA 269 0.0043 10.0240 11.4400
3843 CA ARG 270 0.0034 9.6470 10.0700
3867 CA ILE 271 0.0018 8.9559 7.9100
3886 CA VAL 272 0.0019 9.0109 8.7400
3902 CA ILE 273 0.0016 8.8804 7.6000
3921 CA PRO 274 0.0023 9.1873 8.6800
3935 CA GLY 275 0.0030 9.4608 8.7200
3942 CA ASP 276 0.0038 9.8186 9.8300
3943 CA ASP 276 0.0038 9.8182 10.6200
3961 CA TYR 277 0.0017 8.9417 7.9900
3982 CA ILE 278 0.0017 8.9387 7.0800
4001 CA ASP 279 0.0027 9.3324 8.6900
4013 CA PHE 280 0.0032 9.5393 8.4300
4033 CA GLY 281 0.0093 12.0925 9.5100
4040 CA PRO 282 0.0133 13.7530 9.1200
4054 CA ILE 283 0.0175 15.5012 10.5700
4073 CA SER 284 0.0355 23.0373 12.5100
4084 CA THR 285 0.0678 36.4924 15.3300
4098 CA GLY 286 0.0765 40.1329 12.9800
4105 CA SER 287 0.0267 19.3497 8.2600
4116 CA SER 288 0.0246 18.4804 10.3700
4127 CA SER 289 0.0116 13.0364 8.6700
4128 CA SER 289 0.0116 13.0480 8.7000
4146 CA CYS 290 0.0074 11.3034 8.0800
4156 CA PHE 291 0.0047 10.1682 7.9600
4176 CA GLY 292 0.0029 9.4395 7.3100
4183 CA GLY 293 0.0019 8.9938 7.1000
4190 CA ILE 294 0.0016 8.9019 7.2300
4209 CA GLN 295 0.0028 9.3669 7.4600
4226 CA SER 296 0.0036 9.7356 7.6400
4237 CA SER 297 0.0045 10.1031 8.9900
4249 CA ALA 298 0.0116 13.0398 11.3200
4250 CA ALA 298 0.0116 13.0406 13.7600
4269 CA GLY 299 0.0211 17.0216 18.2300
4270 CA GLY 299 0.0198 16.4670 18.8000
4282 CA ILE 300 0.0111 12.8676 22.2900
4302 CA GLY 301 0.0135 13.8508 22.1600
4309 CA ILE 302 0.0055 10.5005 16.4700
4328 CA ASN 303 0.0024 9.2023 8.9400
4342 CA ILE 304 0.0020 9.0673 7.8300
4361 CA PHE 305 0.0012 8.7263 7.7500
4381 CA GLY 306 0.0015 8.8287 7.9400
4388 CA ASP 307 0.0015 8.8277 7.9300
4400 CA VAL 308 0.0012 8.7141 7.6100
4416 CA ALA 309 0.0010 8.6519 6.8300
4426 CA LEU 310 0.0010 8.6520 7.6500
4446 CA LYS 311 0.0016 8.8771 7.5300
4447 CA LYS 311 0.0016 8.8791 7.7800
4489 CA ALA 312 0.0016 8.8661 7.5400
4500 CA ALA 313 0.0018 8.9675 7.9100
4510 CA PHE 314 0.0017 8.9048 7.8900
4530 CA VAL 315 0.0012 8.7320 7.5900
4546 CA VAL 316 0.0013 8.7720 8.3600
4562 CA PHE 317 0.0013 8.7702 7.7400
4582 CA ASN 318 0.0018 8.9750 9.7700
4596 CA GLY 319 0.0032 9.5325 13.1900
4603 CA ALA 320 0.0060 10.7291 17.3600
4613 CA THR 321 0.0276 19.7363 22.5100
4627 CA THR 322 0.0135 13.8401 18.3200
4641 CA PRO 323 0.0034 9.6482 12.2900
4642 CA PRO 323 0.0034 9.6482 11.9600
4665 CA THR 324 0.0028 9.3859 9.5100
4679 CA LEU 325 0.0016 8.8743 8.8700
4680 CA LEU 325 0.0016 8.8744 9.3500
4714 CA GLY 326 0.0016 8.9011 8.1700
4721 CA PHE 327 0.0015 8.8571 7.6700
4741 CA ALA 328 0.0021 9.1000 8.8900
4752 CA SER 329 0.0034 9.6308 9.4900
4753 CA SER 329 0.0034 9.6231 9.7400
4773 CA LYS 330 0.0040 9.8658 10.8900

If you find results from this site helpful for your research, please cite one of our papers:

K. Suhre & Y.H. Sanejouand, ElNemo: a normal mode web-server for protein movement analysis and the generation of templates for molecular replacement. Nucleic Acids Research, 32, W610-W614, 2004.
K. Suhre & Y.H. Sanejouand, On the potential of normal mode analysis for solving difficult molecular replacement problems. Acta Cryst. D vol.60, p796-799, 2004 © International Union of Crystallography.

elNémo is maintained by Yves-Henri Sanejouand.
It was developed by Karsten Suhre.
Between 2003 and 2014, it was hosted by IGS (Marseille).
Between 2015 and 2025, it was hosted by US2B (Nantes).
Last modification: december 26th, 2025.

Should you encounter any unexpected behaviour, please let us know. elNémo has been hacked on november 27th. It has been moved away and runs again. **Still some additional cleaning from time to time** Sorry for the inconvenience.