Should you encounter any unexpected behaviour,
please let us know.
elNémo has been hacked on november 27th.
It has been moved away and runs again.
Still some additional cleaning from time to time
Sorry for the inconvenience.

* LYASE (OXO-ACID) 04-MAR-94 1CRM *

B-factor analysis for ID 260221185340572269

Overall B-factor prediction

Correlation= 0.484 for 256 C-alpha atoms.

B-factors for all residues

Residue-by-residue B-factor prediction for CA atoms

B-factors are computed based on the first 100 normal modes (B-PREDICT) and are scaled to match the overall B-factors in the submitted model (B-SCALED). A high correlation with the observations is an indicator that the normal modes capture well the overall flexibility features of the model. Comparison of the observed (B-OBS, blue) and the computed (B-SCALED, red) B-factors for all C-alpha atoms is presented below. Differences between B-factors from normal mode analysis and from X-ray crystallographic data may for example indicate regions where the protein flexibility is modified due to external factors such as tight crystall-packing.

ATOM TYP RES RESID B-PREDICT B-SCALED B-OBS	B-SCALED	B-OBS
2 CA TRP 5 0.0254 14.7003 19.1800
16 CA GLY 6 0.0223 13.9622 18.6700
20 CA TYR 7 0.0177 12.8546 19.1400
32 CA ASP 8 0.0380 17.7443 26.4900
40 CA ASP 9 0.0953 31.6112 29.8600
48 CA LYS 10 0.0852 29.1517 30.7000
57 CA ASN 11 0.0381 17.7649 28.8200
65 CA GLY 12 0.0230 14.1175 24.8200
69 CA PRO 13 0.0169 12.6410 21.3400
76 CA GLU 14 0.0367 17.4317 24.5600
85 CA GLN 15 0.0334 16.6461 24.8900
94 CA TRP 16 0.0197 13.3276 23.4500
108 CA SER 17 0.0355 17.1534 26.4700
114 CA LYS 18 0.0631 23.8097 27.4900
123 CA LEU 19 0.0457 19.6128 27.5400
131 CA TYR 20 0.0304 15.9208 26.0800
143 CA PRO 21 0.0383 17.8133 24.5800
150 CA ILE 22 0.0214 13.7463 22.4600
158 CA ALA 23 0.0156 12.3328 20.2600
163 CA ASN 24 0.0283 15.4076 20.0800
171 CA GLY 25 0.0247 14.5417 17.8200
175 CA ASN 26 0.0251 14.6235 15.9500
183 CA ASN 27 0.0114 11.3169 12.8200
191 CA GLN 28 0.0085 10.6239 9.5600
200 CA SER 29 0.0052 9.8187 8.6200
206 CA PRO 30 0.0053 9.8418 7.7000
213 CA VAL 31 0.0086 10.6444 7.6900
220 CA ASP 32 0.0103 11.0448 9.6800
228 CA ILE 33 0.0120 11.4754 12.5400
236 CA LYS 34 0.0219 13.8686 16.5900
245 CA THR 35 0.0313 16.1276 20.2200
252 CA SER 36 0.0709 25.6925 23.4500
258 CA GLU 37 0.0431 18.9757 24.6000
267 CA THR 38 0.0288 15.5373 23.2600
274 CA LYS 39 0.0343 16.8658 21.7600
283 CA HIS 40 0.0306 15.9648 18.1400
293 CA ASP 41 0.0356 17.1741 17.3700
301 CA THR 42 0.0698 25.4330 15.4100
308 CA SER 43 0.0519 21.1102 18.3400
314 CA LEU 44 0.0251 14.6300 19.6500
322 CA LYS 45 0.0284 15.4317 21.5700
331 CA PRO 46 0.0263 14.9260 21.7400
338 CA ILE 47 0.0153 12.2683 21.4900
346 CA SER 48 0.0192 13.2137 19.1500
352 CA VAL 49 0.0150 12.1838 16.7100
359 CA SER 50 0.0174 12.7686 15.0200
365 CA TYR 51 0.0110 11.2291 12.2400
377 CA ASN 52 0.0153 12.2665 13.9400
385 CA PRO 53 0.0186 13.0526 13.5100
392 CA ALA 54 0.0221 13.8974 12.4300
397 CA THR 55 0.0127 11.6247 10.6900
404 CA ALA 56 0.0119 11.4452 9.5100
409 CA LYS 57 0.0149 12.1766 12.5300
418 CA GLU 58 0.0142 11.9900 12.2900
427 CA ILE 59 0.0106 11.1238 9.4400
435 CA ILE 60 0.0114 11.3121 8.6400
443 CA ASN 61 0.0107 11.1616 7.8500
451 CA VAL 62 0.0140 11.9436 10.6700
458 CA GLY 63 0.0124 11.5724 11.7400
462 CA HIS 64 0.0097 10.9006 10.0500
472 CA SER 65 0.0067 10.1954 7.9400
478 CA PHE 66 0.0058 9.9755 6.0000
489 CA HIS 67 0.0071 10.2925 6.7000
499 CA VAL 68 0.0070 10.2491 8.7500
506 CA ASN 69 0.0085 10.6291 10.7200
514 CA PHE 70 0.0083 10.5805 12.6900
525 CA GLU 71 0.0148 12.1411 17.0500
534 CA ASP 72 0.0135 11.8175 19.1700
542 CA ASN 73 0.0467 19.8459 23.5100
550 CA GLN 74 0.0357 17.2056 24.8500
559 CA ASP 75 0.0185 13.0364 21.8400
567 CA ARG 76 0.0198 13.3542 18.7100
578 CA SER 77 0.0095 10.8737 13.3400
584 CA VAL 78 0.0096 10.8847 12.3000
591 CA LEU 79 0.0090 10.7521 11.8000
599 CA LYS 80 0.0129 11.6918 15.1800
608 CA GLY 81 0.0168 12.6251 13.2000
612 CA GLY 82 0.0153 12.2601 14.8600
616 CA PRO 83 0.0155 12.3044 15.7000
623 CA PHE 84 0.0146 12.0863 16.6600
634 CA SER 85 0.0521 21.1555 21.0300
640 CA ASP 86 0.0214 13.7442 21.3600
648 CA SER 87 0.0101 11.0148 17.0200
654 CA TYR 88 0.0074 10.3598 12.8600
666 CA ARG 89 0.0076 10.4102 10.8600
677 CA LEU 90 0.0061 10.0450 6.7700
685 CA PHE 91 0.0063 10.0862 4.8400
696 CA GLN 92 0.0061 10.0313 6.6800
705 CA PHE 93 0.0049 9.7433 7.3400
716 CA HIS 94 0.0048 9.7259 5.6000
726 CA PHE 95 0.0039 9.5166 4.0400
737 CA HIS 96 0.0041 9.5489 5.0100
747 CA TRP 97 0.0058 9.9634 6.5100
761 CA GLY 98 0.0073 10.3367 8.0800
765 CA SER 99 0.0196 13.3080 10.8100
771 CA THR 100 0.0229 14.0965 11.1700
778 CA ASN 101 0.0154 12.2923 11.3500
786 CA GLU 102 0.0198 13.3431 12.4700
795 CA HIS 103 0.0175 12.8028 12.5700
805 CA GLY 104 0.0087 10.6760 8.2200
809 CA SER 105 0.0056 9.9271 7.0800
815 CA GLU 106 0.0049 9.7419 7.6000
824 CA HIS 107 0.0057 9.9405 7.2400
834 CA THR 108 0.0083 10.5666 8.3300
841 CA VAL 109 0.0152 12.2513 11.0400
848 CA ASP 110 0.0303 15.8829 14.7000
856 CA GLY 111 0.0389 17.9719 14.0400
860 CA VAL 112 0.0318 16.2635 14.3800
867 CA LYS 113 0.0104 11.0764 13.2400
876 CA TYR 114 0.0096 10.8976 11.0500
888 CA SER 115 0.0076 10.4001 8.6200
894 CA ALA 116 0.0060 10.0068 5.5800
899 CA GLU 117 0.0041 9.5592 4.4600
908 CA LEU 118 0.0043 9.5962 4.7800
916 CA HIS 119 0.0037 9.4667 4.4300
926 CA VAL 120 0.0042 9.5834 5.6200
933 CA ALA 121 0.0059 9.9857 5.9800
938 CA HIS 122 0.0054 9.8702 6.7100
948 CA TRP 123 0.0088 10.6815 10.7200
962 CA ASN 124 0.0098 10.9320 14.3200
970 CA SER 125 0.0139 11.9203 18.5700
976 CA ALA 126 0.0215 13.7550 23.4100
981 CA LYS 127 0.0288 15.5242 24.6500
990 CA TYR 128 0.0245 14.4786 24.4600
1002 CA SER 129 0.0480 20.1785 24.5700
1008 CA SER 130 0.0352 17.0651 21.5000
1014 CA LEU 131 0.0207 13.5656 17.8600
1022 CA ALA 132 0.0363 17.3423 20.4600
1027 CA GLU 133 0.0360 17.2608 23.0300
1036 CA ALA 134 0.0183 12.9968 19.8900
1041 CA ALA 135 0.0197 13.3377 17.7600
1046 CA SER 136 0.0231 14.1495 16.6200
1052 CA LYS 137 0.0238 14.3237 16.3500
1061 CA ALA 138 0.0239 14.3397 13.8200
1066 CA ASP 139 0.0170 12.6695 11.6500
1074 CA GLY 140 0.0108 11.1877 10.1800
1078 CA LEU 141 0.0063 10.0857 8.4300
1086 CA ALA 142 0.0056 9.9265 5.9700
1091 CA VAL 143 0.0042 9.5867 5.3100
1098 CA ILE 144 0.0042 9.5790 6.0600
1106 CA GLY 145 0.0047 9.7026 6.6200
1110 CA VAL 146 0.0052 9.8174 6.4300
1117 CA LEU 147 0.0063 10.0792 8.4400
1125 CA MET 148 0.0063 10.0976 8.2600
1133 CA LYS 149 0.0133 11.7779 11.7400
1142 CA VAL 150 0.0158 12.3763 12.9500
1149 CA GLY 151 0.0487 20.3349 15.0900
1153 CA GLU 152 0.0645 24.1476 19.0300
1162 CA ALA 153 0.0290 15.5686 16.4000
1167 CA ASN 154 0.0210 13.6351 14.8400
1175 CA PRO 155 0.0303 15.8886 14.5500
1182 CA LYS 156 0.0242 14.4090 14.7800
1191 CA LEU 157 0.0143 12.0291 12.5900
1199 CA GLN 158 0.0199 13.3797 14.7800
1208 CA LYS 159 0.0178 12.8657 13.9400
1217 CA VAL 160 0.0109 11.1960 12.4000
1224 CA LEU 161 0.0143 12.0110 12.5900
1232 CA ASP 162 0.0240 14.3578 13.9500
1240 CA ALA 163 0.0150 12.1981 14.2500
1245 CA LEU 164 0.0121 11.4999 15.1400
1253 CA GLN 165 0.0369 17.4749 17.9700
1262 CA ALA 166 0.0269 15.0655 14.3100
1267 CA ILE 167 0.0139 11.9330 12.7300
1275 CA LYS 168 0.0146 12.0914 13.1500
1284 CA THR 169 0.0203 13.4797 10.1700
1291 CA LYS 170 0.0226 14.0330 12.1700
1300 CA GLY 171 0.0313 16.1390 12.4500
1304 CA LYS 172 0.0260 14.8402 13.6500
1313 CA ARG 173 0.0236 14.2583 15.8100
1324 CA ALA 174 0.0285 15.4563 14.2700
1329 CA PRO 175 0.0311 16.0781 13.4000
1336 CA PHE 176 0.0169 12.6428 12.4600
1347 CA THR 177 0.0237 14.3047 14.9900
1354 CA ASN 178 0.0272 15.1408 15.3800
1362 CA PHE 179 0.0126 11.6047 12.3600
1373 CA ASP 180 0.0117 11.4004 12.3600
1381 CA PRO 181 0.0093 10.8033 11.2600
1388 CA SER 182 0.0124 11.5597 12.4700
1394 CA THR 183 0.0157 12.3698 13.4400
1401 CA LEU 184 0.0126 11.5995 12.4000
1409 CA LEU 185 0.0139 11.9190 13.5100
1417 CA PRO 186 0.0190 13.1577 16.2200
1424 CA SER 187 0.0668 24.7243 21.1200
1430 CA SER 188 0.0345 16.9138 20.6100
1436 CA LEU 189 0.0248 14.5508 19.5700
1444 CA ASP 190 0.0166 12.5708 16.1500
1452 CA PHE 191 0.0081 10.5288 11.2900
1463 CA TRP 192 0.0074 10.3664 8.7800
1477 CA THR 193 0.0058 9.9762 7.6300
1484 CA TYR 194 0.0056 9.9085 7.3000
1496 CA PRO 195 0.0076 10.4057 6.7700
1503 CA GLY 196 0.0064 10.1153 7.3800
1507 CA SER 197 0.0064 10.1128 6.9000
1513 CA LEU 198 0.0072 10.3016 7.2200
1521 CA THR 199 0.0053 9.8385 7.3800
1528 CA HIS 200 0.0065 10.1269 10.2700
1538 CA PRO 201 0.0134 11.8062 10.5200
1545 CA PRO 202 0.0172 12.7232 12.1900
1552 CA LEU 203 0.0093 10.8241 11.7400
1560 CA TYR 204 0.0129 11.6811 11.6800
1572 CA GLU 205 0.0090 10.7469 10.9300
1581 CA SER 206 0.0107 11.1591 8.6500
1587 CA VAL 207 0.0062 10.0630 5.7100
1594 CA THR 208 0.0057 9.9456 6.7500
1601 CA TRP 209 0.0038 9.4892 6.0400
1615 CA ILE 210 0.0043 9.6070 7.9900
1623 CA ILE 211 0.0051 9.8100 8.7700
1631 CA CYS 212 0.0066 10.1670 11.8900
1637 CA LYS 213 0.0135 11.8242 13.1100
1646 CA GLU 214 0.0156 12.3453 14.6800
1655 CA SER 215 0.0113 11.2942 12.7200
1661 CA ILE 216 0.0111 11.2422 10.2200
1669 CA SER 217 0.0189 13.1276 12.0800
1675 CA VAL 218 0.0142 11.9971 10.4800
1682 CA SER 219 0.0209 13.6107 11.5800
1688 CA SER 220 0.0208 13.5871 12.0100
1694 CA GLU 221 0.0345 16.9096 13.3100
1703 CA GLN 222 0.0155 12.3179 9.1500
1712 CA LEU 223 0.0099 10.9512 9.6900
1720 CA ALA 224 0.0132 11.7559 10.2500
1725 CA GLN 225 0.0097 10.9017 11.2000
1734 CA PHE 226 0.0079 10.4729 10.3800
1745 CA ARG 227 0.0093 10.8202 12.0500
1756 CA SER 228 0.0135 11.8408 14.2900
1762 CA LEU 229 0.0113 11.2892 12.0000
1770 CA LEU 230 0.0149 12.1575 10.2500
1778 CA SER 231 0.0229 14.0989 11.1700
1784 CA ASN 232 0.0347 16.9473 10.6500
1792 CA VAL 233 0.0593 22.8981 13.5100
1799 CA GLU 234 0.0881 29.8619 17.2900
1808 CA GLY 235 0.2129 60.0183 16.6400
1812 CA ASP 236 0.1404 42.5065 15.1500
1820 CA ASN 237 0.1505 44.9366 12.0600
1828 CA ALA 238 0.0989 32.4733 13.2200
1833 CA VAL 239 0.0446 19.3553 12.5200
1840 CA PRO 240 0.0255 14.7221 11.8900
1847 CA MET 241 0.0099 10.9661 11.6900
1855 CA GLN 242 0.0121 11.4838 14.2500
1864 CA HIS 243 0.0091 10.7618 11.7400
1874 CA ASN 244 0.0073 10.3270 8.6200
1882 CA ASN 245 0.0061 10.0404 8.3000
1890 CA ARG 246 0.0047 9.7124 9.0800
1901 CA PRO 247 0.0086 10.6459 10.9400
1908 CA THR 248 0.0081 10.5305 12.0500
1915 CA GLN 249 0.0126 11.6145 12.1200
1924 CA PRO 250 0.0210 13.6476 13.6000
1931 CA LEU 251 0.0190 13.1673 15.0300
1939 CA LYS 252 0.0308 16.0058 16.8000
1948 CA GLY 253 0.0687 25.1818 15.9900
1952 CA ARG 254 0.0325 16.4149 13.0400
1963 CA THR 255 0.0221 13.9070 13.4100
1970 CA VAL 256 0.0123 11.5322 12.8300
1977 CA ARG 257 0.0136 11.8487 13.1500
1988 CA ALA 258 0.0140 11.9390 12.6300
1993 CA SER 259 0.0176 12.8189 13.5300
1999 CA PHE 260 0.0293 15.6492 15.5000

If you find results from this site helpful for your research, please cite one of our papers:

K. Suhre & Y.H. Sanejouand, ElNemo: a normal mode web-server for protein movement analysis and the generation of templates for molecular replacement. Nucleic Acids Research, 32, W610-W614, 2004.
K. Suhre & Y.H. Sanejouand, On the potential of normal mode analysis for solving difficult molecular replacement problems. Acta Cryst. D vol.60, p796-799, 2004 © International Union of Crystallography.

elNémo is maintained by Yves-Henri Sanejouand.
It was developed by Karsten Suhre.
Between 2003 and 2014, it was hosted by IGS (Marseille).
Between 2015 and 2025, it was hosted by US2B (Nantes).
Last modification: december 26th, 2025.

Should you encounter any unexpected behaviour, please let us know. elNémo has been hacked on november 27th. It has been moved away and runs again. **Still some additional cleaning from time to time** Sorry for the inconvenience.