Should you encounter any unexpected behaviour,
please let us know.
elNémo has been hacked on november 27th.
It has been moved away and runs again.
Still some additional cleaning from time to time
Sorry for the inconvenience.

* MADHURJA truncatula medicago single *

B-factor analysis for ID 260222211152815844

Overall B-factor prediction

Correlation= -0.170 for 192 C-alpha atoms.

B-factors for all residues

Residue-by-residue B-factor prediction for CA atoms

B-factors are computed based on the first 100 normal modes (B-PREDICT) and are scaled to match the overall B-factors in the submitted model (B-SCALED). A high correlation with the observations is an indicator that the normal modes capture well the overall flexibility features of the model. Comparison of the observed (B-OBS, blue) and the computed (B-SCALED, red) B-factors for all C-alpha atoms is presented below. Differences between B-factors from normal mode analysis and from X-ray crystallographic data may for example indicate regions where the protein flexibility is modified due to external factors such as tight crystall-packing.

ATOM TYP RES RESID B-PREDICT B-SCALED B-OBS	B-SCALED	B-OBS
2 CA MET 1 0.2009 92.6477 61.4400
10 CA ASP 2 0.2544 92.8956 83.5000
18 CA LEU 3 0.1934 92.6133 83.4400
26 CA ARG 4 0.2556 92.9013 88.1200
37 CA GLU 5 0.2229 92.7498 89.5000
46 CA SER 6 0.1460 92.3934 90.4400
52 CA ILE 7 0.1744 92.5253 91.9400
60 CA ALA 8 0.2251 92.7601 94.0000
65 CA ASN 9 0.1638 92.4762 93.1900
73 CA GLN 10 0.1255 92.2988 94.0000
82 CA THR 11 0.1911 92.6024 95.0000
89 CA ASN 12 0.2056 92.6695 94.6200
97 CA VAL 13 0.1385 92.3589 93.5600
104 CA SER 14 0.1525 92.4237 95.3100
110 CA LEU 15 0.2252 92.7604 94.9400
118 CA SER 16 0.2085 92.6833 94.2500
124 CA VAL 17 0.1632 92.4735 94.1200
131 CA ALA 18 0.1951 92.6212 93.9400
136 CA LYS 19 0.2675 92.9565 93.3100
145 CA HIS 20 0.2783 93.0062 92.6200
155 CA LEU 21 0.2523 92.8860 91.2500
163 CA PHE 22 0.2960 93.0884 89.9400
174 CA SER 23 0.4151 93.6401 91.3100
180 CA LYS 24 0.5133 94.0946 87.6200
189 CA GLU 25 0.5370 94.2046 82.1200
198 CA SER 26 0.7347 95.1202 77.0000
204 CA ASP 27 0.8078 95.4588 83.8100
212 CA ASN 28 0.7051 94.9832 87.7500
220 CA ASN 29 0.6423 94.6923 90.0600
228 CA ILE 30 0.4054 93.5949 91.5600
236 CA VAL 31 0.4247 93.6843 91.8100
243 CA PHE 32 0.2375 92.8174 92.3100
254 CA SER 33 0.1263 92.3022 95.0000
260 CA PRO 34 0.1060 92.2083 94.8100
267 CA LEU 35 0.0827 92.1006 94.3800
275 CA SER 36 0.0603 91.9969 94.4400
281 CA LEU 37 0.0552 91.9731 94.3100
289 CA GLN 38 0.0481 91.9401 94.6200
298 CA VAL 39 0.0287 91.8503 95.5000
305 CA VAL 40 0.0217 91.8181 96.0600
312 CA LEU 41 0.0278 91.8464 96.1200
320 CA SER 42 0.0252 91.8339 95.8800
326 CA ILE 43 0.0170 91.7959 95.9400
334 CA ILE 44 0.0275 91.8450 96.3100
342 CA ALA 45 0.0416 91.9102 96.6900
347 CA SER 46 0.0442 91.9221 94.8800
353 CA GLY 47 0.0586 91.9887 94.1900
357 CA SER 48 0.0922 92.1445 95.4400
363 CA GLU 49 0.1792 92.5472 94.7500
372 CA GLY 50 0.2475 92.8638 96.4400
376 CA PRO 51 0.2399 92.8283 97.6900
383 CA THR 52 0.1423 92.3764 97.5000
390 CA GLN 53 0.0959 92.1614 96.6900
399 CA GLN 54 0.1373 92.3534 96.8800
408 CA GLN 55 0.1308 92.3232 97.3800
417 CA LEU 56 0.0726 92.0535 97.5000
425 CA PHE 57 0.0923 92.1447 95.6900
436 CA ASN 58 0.1340 92.3381 95.7500
444 CA PHE 59 0.1043 92.2005 95.9400
455 CA LEU 60 0.0824 92.0992 94.5000
463 CA GLN 61 0.1259 92.3003 93.4400
472 CA SER 62 0.1080 92.2177 91.5600
478 CA LYS 63 0.1429 92.3790 87.4400
487 CA SER 64 0.1106 92.2296 91.9400
493 CA THR 65 0.0714 92.0480 92.1900
500 CA ASP 66 0.0906 92.1369 91.5600
508 CA HIS 67 0.0899 92.1338 91.4400
518 CA LEU 68 0.0531 91.9634 92.6900
526 CA ASN 69 0.0433 91.9181 93.0000
534 CA TYR 70 0.0666 92.0258 92.3100
546 CA PHE 71 0.0685 92.0346 92.8100
557 CA ALA 72 0.0426 91.9148 92.6900
562 CA SER 73 0.0468 91.9343 92.8800
568 CA GLN 74 0.0791 92.0839 93.3100
577 CA LEU 75 0.0724 92.0529 93.5000
585 CA VAL 76 0.0549 91.9715 92.8800
592 CA SER 77 0.0787 92.0819 91.4400
598 CA VAL 78 0.1303 92.3208 90.6200
605 CA ILE 79 0.1281 92.3107 91.3100
613 CA LEU 80 0.0975 92.1688 93.3800
621 CA SER 81 0.1199 92.2728 93.1200
627 CA ASP 82 0.1736 92.5216 94.3100
635 CA ALA 83 0.2480 92.8659 94.0600
640 CA SER 84 0.3220 93.2088 93.8800
646 CA PRO 85 0.5406 94.2212 93.8800
653 CA ALA 86 0.6101 94.5430 93.4400
658 CA GLY 87 0.6836 94.8836 92.8800
662 CA GLY 88 0.4092 93.6127 94.1200
666 CA PRO 89 0.2796 93.0126 95.5600
673 CA LEU 90 0.2262 92.7649 95.1900
681 CA LEU 91 0.1636 92.4750 94.9400
689 CA SER 92 0.1295 92.3172 94.8800
695 CA PHE 93 0.0838 92.1055 94.8100
706 CA VAL 94 0.0640 92.0139 93.6200
713 CA ASP 95 0.0361 91.8847 95.3100
721 CA GLY 96 0.0333 91.8716 96.0600
725 CA VAL 97 0.0331 91.8707 97.3800
732 CA TRP 98 0.0567 91.9798 97.8100
746 CA VAL 99 0.0814 92.0943 97.8100
753 CA ASP 100 0.1294 92.3169 98.3100
761 CA GLN 101 0.1862 92.5799 97.2500
770 CA THR 102 0.2425 92.8407 96.9400
777 CA LEU 103 0.1908 92.6012 96.0000
785 CA SER 104 0.2231 92.7509 93.1900
791 CA LEU 105 0.1330 92.3334 93.5000
799 CA GLN 106 0.1667 92.4893 94.4400
808 CA PRO 107 0.1789 92.5460 95.1900
815 CA SER 108 0.1448 92.3878 93.5000
821 CA PHE 109 0.0718 92.0498 93.4400
832 CA GLN 110 0.0715 92.0487 94.5000
841 CA GLN 111 0.0885 92.1272 93.7500
850 CA ILE 112 0.0477 91.9381 91.9400
858 CA VAL 113 0.0280 91.8469 93.8800
865 CA SER 114 0.0445 91.9237 92.5000
871 CA THR 115 0.0535 91.9654 88.8100
878 CA HIS 116 0.0357 91.8829 87.3100
888 CA PHE 117 0.0231 91.8244 92.1200
899 CA LYS 118 0.0352 91.8803 93.0000
908 CA ALA 119 0.0278 91.8460 95.5000
913 CA ALA 120 0.0420 91.9121 95.6900
918 CA LEU 121 0.0502 91.9499 95.8800
926 CA SER 122 0.0805 92.0903 96.6900
932 CA SER 123 0.1180 92.2640 97.4400
938 CA VAL 124 0.1384 92.3586 98.0000
945 CA ASP 125 0.1940 92.6157 98.0000
953 CA PHE 126 0.1679 92.4951 98.2500
964 CA GLN 127 0.2439 92.8472 97.5600
973 CA ASN 128 0.3137 93.1702 98.0000
981 CA LYS 129 0.2602 92.9224 97.8800
990 CA ALA 130 0.2038 92.6615 97.3100
995 CA VAL 131 0.2559 92.9027 97.3800
1002 CA GLU 132 0.2499 92.8748 97.9400
1011 CA VAL 133 0.1584 92.4509 98.0000
1018 CA THR 134 0.1667 92.4894 97.8100
1025 CA ASN 135 0.2125 92.7014 97.3800
1033 CA GLU 136 0.1615 92.4654 97.4400
1042 CA VAL 137 0.1131 92.2413 97.8100
1049 CA ASN 138 0.1621 92.4681 97.8800
1057 CA SER 139 0.1892 92.5935 97.3100
1063 CA TRP 140 0.1266 92.3037 97.5000
1077 CA ALA 141 0.1204 92.2749 96.5600
1082 CA GLU 142 0.1875 92.5859 97.0000
1091 CA LYS 143 0.1894 92.5945 96.0000
1100 CA GLU 144 0.1314 92.3259 92.9400
1109 CA THR 145 0.1575 92.4467 93.2500
1116 CA ASN 146 0.2396 92.8271 92.6200
1124 CA GLY 147 0.2785 93.0074 94.0600
1128 CA LEU 148 0.2533 92.8905 92.7500
1136 CA ILE 149 0.2048 92.6660 92.3100
1144 CA LYS 150 0.2499 92.8750 92.7500
1153 CA GLU 151 0.2323 92.7932 94.5000
1162 CA LEU 152 0.1551 92.4357 94.8800
1170 CA LEU 153 0.1650 92.4816 96.1900
1178 CA PRO 154 0.2635 92.9379 95.1900
1185 CA LEU 155 0.3120 93.1626 92.4400
1193 CA GLY 156 0.3082 93.1450 93.5000
1197 CA SER 157 0.2308 92.7863 95.3800
1203 CA VAL 158 0.1731 92.5192 97.0600
1210 CA ASN 159 0.2233 92.7514 97.1900
1218 CA ASN 160 0.2436 92.8455 97.1900
1226 CA ALA 161 0.2336 92.7991 97.5000
1231 CA THR 162 0.1513 92.4179 98.0600
1238 CA ARG 163 0.1263 92.3023 97.4400
1249 CA LEU 164 0.0723 92.0523 97.0000
1257 CA ILE 165 0.0573 91.9827 97.5600
1265 CA PHE 166 0.0338 91.8738 96.7500
1276 CA ALA 167 0.0522 91.9593 96.0000
1281 CA ASN 168 0.0544 91.9691 95.5600
1289 CA ALA 169 0.0920 92.1436 94.1900
1294 CA LEU 170 0.1151 92.2506 95.5000
1302 CA TYR 171 0.1764 92.5345 95.3800
1314 CA PHE 172 0.2198 92.7352 95.3100
1325 CA LYS 173 0.2897 93.0590 95.4400
1334 CA GLY 174 0.3851 93.5011 96.0000
1338 CA ALA 175 0.4690 93.8895 95.6200
1343 CA TRP 176 0.7238 95.0695 95.5600
1357 CA ASN 177 1.9343 100.6761 94.7500
1365 CA ASP 178 2.1967 101.8916 96.0000
1373 CA LYS 179 1.2512 97.5122 95.6900
1382 CA PHE 180 1.7695 99.9127 94.8800
1393 CA ASP 181 2.9646 105.4479 95.3100
1401 CA ALA 182 3.5381 108.1044 93.8800
1406 CA SER 183 5.4739 117.0699 95.8100
1412 CA LYS 184 4.2300 111.3088 95.0600
1421 CA ARG 185 3.5261 108.0485 92.8100
1432 CA LYS 186 4.6173 113.1026 94.0600
1441 CA THR 187 4.9363 114.5800 93.6200
1448 CA THR 188 4.6406 113.2104 92.8100
1455 CA SER 189 4.8390 114.1293 91.5000
1461 CA PHE 190 2.9975 105.6002 88.5000
1472 CA THR 191 3.3398 107.1857 83.0000
1479 CA PHE 192 2.6532 104.0060 64.1900

If you find results from this site helpful for your research, please cite one of our papers:

K. Suhre & Y.H. Sanejouand, ElNemo: a normal mode web-server for protein movement analysis and the generation of templates for molecular replacement. Nucleic Acids Research, 32, W610-W614, 2004.
K. Suhre & Y.H. Sanejouand, On the potential of normal mode analysis for solving difficult molecular replacement problems. Acta Cryst. D vol.60, p796-799, 2004 © International Union of Crystallography.

elNémo is maintained by Yves-Henri Sanejouand.
It was developed by Karsten Suhre.
Between 2003 and 2014, it was hosted by IGS (Marseille).
Between 2015 and 2025, it was hosted by US2B (Nantes).
Last modification: december 26th, 2025.

Should you encounter any unexpected behaviour, please let us know. elNémo has been hacked on november 27th. It has been moved away and runs again. **Still some additional cleaning from time to time** Sorry for the inconvenience.