Should you encounter any unexpected behaviour,
please let us know.
elNémo has been hacked on november 27th.
It has been moved away and runs again.
Still some additional cleaning from time to time
Sorry for the inconvenience.

* MEMBRANE PROTEIN 26-MAR-24 8YU7 *

B-factor analysis for ID 2603180307243691338

Overall B-factor prediction

Correlation= 0.667 for 280 C-alpha atoms.

B-factors for all residues

Residue-by-residue B-factor prediction for CA atoms

B-factors are computed based on the first 100 normal modes (B-PREDICT) and are scaled to match the overall B-factors in the submitted model (B-SCALED). A high correlation with the observations is an indicator that the normal modes capture well the overall flexibility features of the model. Comparison of the observed (B-OBS, blue) and the computed (B-SCALED, red) B-factors for all C-alpha atoms is presented below. Differences between B-factors from normal mode analysis and from X-ray crystallographic data may for example indicate regions where the protein flexibility is modified due to external factors such as tight crystall-packing.

ATOM TYP RES RESID B-PREDICT B-SCALED B-OBS	B-SCALED	B-OBS
2 CA GLU 26 0.9245 198.6370 73.5600
11 CA PRO 27 0.5226 113.7290 73.3500
18 CA CYS 28 0.2023 46.0747 72.5400
24 CA PHE 29 0.2000 45.5952 73.1000
35 CA ARG 30 0.1210 28.9129 71.9000
46 CA GLU 31 0.0792 20.0640 69.2100
55 CA GLU 32 0.0683 17.7749 63.2900
64 CA ASN 33 0.0506 14.0436 37.5200
72 CA ALA 34 0.0786 19.9418 22.8300
77 CA ASN 35 0.0868 21.6720 14.8000
85 CA PHE 36 0.0751 19.2089 10.3700
96 CA ASN 37 0.0404 11.8770 11.0300
104 CA LYS 38 0.0326 10.2278 7.7700
113 CA ILE 39 0.0420 12.2126 4.0400
121 CA PHE 40 0.0323 10.1593 4.1500
132 CA LEU 41 0.0228 8.1590 2.3000
140 CA PRO 42 0.0210 7.7742 1.5500
147 CA THR 43 0.0269 9.0305 1.3500
154 CA ILE 44 0.0177 7.0879 0.9100
162 CA TYR 45 0.0136 6.2128 0.0600
174 CA SER 46 0.0191 7.3799 0.1300
180 CA ILE 47 0.0227 8.1367 0.7100
188 CA ILE 48 0.0139 6.2863 1.1900
196 CA PHE 49 0.0139 6.2876 0.4900
207 CA LEU 50 0.0216 7.8997 0.6500
215 CA THR 51 0.0199 7.5395 1.7500
222 CA GLY 52 0.0138 6.2553 2.2500
226 CA ILE 53 0.0166 6.8445 1.1200
234 CA VAL 54 0.0366 11.0863 2.3600
241 CA GLY 55 0.0264 8.9181 3.4500
245 CA ASN 56 0.0157 6.6703 3.3000
253 CA GLY 57 0.0261 8.8492 2.7900
257 CA LEU 58 0.0410 12.0097 4.9400
265 CA VAL 59 0.0246 8.5366 5.4300
272 CA ILE 60 0.0268 9.0132 5.4000
280 CA LEU 61 0.0507 14.0584 8.5600
288 CA VAL 62 0.0575 15.4913 10.0100
295 CA MET 63 0.0382 11.4241 13.7900
303 CA GLY 64 0.0664 17.3720 16.9900
307 CA TYR 65 0.1046 25.4388 19.2700
319 CA GLN 66 0.1160 27.8435 20.2400
328 CA LYS 67 0.1286 30.5157 28.0200
337 CA LYS 68 0.0770 19.6172 23.4500
346 CA LEU 69 0.0595 15.9175 21.6800
354 CA ARG 70 0.0989 24.2318 26.5700
365 CA SER 71 0.0837 21.0280 17.3500
371 CA MET 72 0.0175 7.0470 14.0000
379 CA THR 73 0.0151 6.5255 10.4300
386 CA ASP 74 0.0192 7.3921 13.5800
394 CA LYS 75 0.0156 6.6296 8.6500
403 CA TYR 76 0.0094 5.3408 6.3000
415 CA ARG 77 0.0110 5.6665 7.6900
426 CA LEU 78 0.0130 6.0814 5.3500
434 CA HIS 79 0.0113 5.7329 5.0100
444 CA LEU 80 0.0097 5.3830 3.2000
452 CA SER 81 0.0107 5.6094 3.2000
458 CA VAL 82 0.0128 6.0572 2.3100
465 CA ALA 83 0.0124 5.9620 2.5600
470 CA ASP 84 0.0111 5.6992 2.3700
478 CA LEU 85 0.0123 5.9387 1.5000
486 CA LEU 86 0.0135 6.2014 1.0100
494 CA PHE 87 0.0113 5.7263 0.5000
505 CA VAL 88 0.0116 5.7986 0.5300
512 CA ILE 89 0.0153 6.5786 0.3700
520 CA THR 90 0.0136 6.2147 0.8500
527 CA LEU 91 0.0129 6.0660 1.1800
535 CA PRO 92 0.0170 6.9372 2.2100
542 CA PHE 93 0.0161 6.7395 5.0300
553 CA TRP 94 0.0148 6.4717 3.4500
567 CA ALA 95 0.0197 7.4954 4.0000
572 CA VAL 96 0.0246 8.5429 6.1900
579 CA ASP 97 0.0220 7.9926 10.8900
587 CA ALA 98 0.0272 9.0934 8.7000
592 CA VAL 99 0.0334 10.3913 7.5800
599 CA ALA 100 0.0475 13.3727 14.2500
604 CA ASN 101 0.0340 10.5365 21.4500
612 CA TRP 102 0.0265 8.9481 22.5000
626 CA TYR 103 0.0368 11.1091 24.1200
638 CA PHE 104 0.0482 13.5347 24.9500
649 CA GLY 105 0.0429 12.3998 22.9900
653 CA ASN 106 0.0329 10.2882 18.3000
661 CA PHE 107 0.0427 12.3643 16.3400
672 CA LEU 108 0.0254 8.7062 12.8900
680 CA CYS 109 0.0172 6.9712 12.7700
686 CA LYS 110 0.0176 7.0604 8.7300
695 CA ALA 111 0.0199 7.5532 6.8400
700 CA VAL 112 0.0150 6.5042 5.9300
707 CA HIS 113 0.0152 6.5553 5.7300
717 CA VAL 114 0.0146 6.4292 3.5500
724 CA ILE 115 0.0134 6.1681 1.9900
732 CA TYR 116 0.0129 6.0598 1.8000
744 CA THR 117 0.0152 6.5463 2.1300
751 CA VAL 118 0.0142 6.3542 1.2600
758 CA ASN 119 0.0121 5.8915 1.6100
766 CA LEU 120 0.0131 6.1118 1.5900
774 CA TYR 121 0.0141 6.3260 1.9800
786 CA SER 122 0.0107 5.5982 1.9800
792 CA SER 123 0.0102 5.4893 1.3300
798 CA VAL 124 0.0105 5.5527 0.9500
805 CA LEU 125 0.0099 5.4376 1.0600
813 CA ILE 126 0.0087 5.1877 1.9300
821 CA LEU 127 0.0087 5.1824 1.3900
829 CA ALA 128 0.0113 5.7363 1.4100
834 CA PHE 129 0.0098 5.4059 2.9000
845 CA ILE 130 0.0087 5.1756 2.5400
853 CA SER 131 0.0100 5.4596 3.1500
859 CA LEU 132 0.0128 6.0563 3.7800
867 CA ASP 133 0.0124 5.9536 7.7100
875 CA ARG 134 0.0131 6.1153 7.8200
886 CA TYR 135 0.0161 6.7419 7.7500
898 CA LEU 136 0.0256 8.7602 8.2000
906 CA ALA 137 0.0292 9.5027 10.4600
911 CA ILE 138 0.0297 9.6284 10.7500
919 CA VAL 139 0.0400 11.7888 11.1800
926 CA HIS 140 0.0754 19.2725 16.1400
936 CA ALA 141 0.0832 20.9236 18.9300
941 CA THR 142 0.1967 44.9005 26.6900
948 CA ASN 143 0.1725 39.7748 27.3500
956 CA SER 144 0.0751 19.2080 26.1200
962 CA GLN 145 0.0759 19.3847 27.3300
971 CA ARG 146 0.0951 23.4403 29.0300
982 CA PRO 147 0.0623 16.4966 22.9100
989 CA ARG 148 0.0288 9.4318 20.4800
1000 CA LYS 149 0.0447 12.7808 21.4300
1009 CA LEU 150 0.0511 14.1488 17.5400
1017 CA LEU 151 0.0339 10.5109 15.3300
1025 CA ALA 152 0.0241 8.4300 16.3600
1030 CA GLU 153 0.0350 10.7360 16.8900
1039 CA LYS 154 0.0506 14.0383 15.2300
1048 CA VAL 155 0.0373 11.2275 12.0300
1055 CA VAL 156 0.0208 7.7292 10.7200
1062 CA TYR 157 0.0332 10.3586 10.8000
1074 CA VAL 158 0.0418 12.1753 10.9900
1081 CA GLY 159 0.0344 10.6116 10.0200
1085 CA VAL 160 0.0169 6.9181 8.3600
1092 CA TRP 161 0.0231 8.2261 8.0500
1106 CA ILE 162 0.0367 11.1039 7.4900
1114 CA PRO 163 0.0298 9.6470 6.4600
1121 CA ALA 164 0.0190 7.3526 5.2400
1126 CA LEU 165 0.0257 8.7815 5.7300
1134 CA LEU 166 0.0327 10.2531 5.5000
1142 CA LEU 167 0.0235 8.3087 4.6100
1150 CA THR 168 0.0205 7.6796 5.3700
1157 CA ILE 169 0.0316 10.0188 6.6600
1165 CA PRO 170 0.0228 8.1533 8.2600
1172 CA ASP 171 0.0176 7.0666 9.1800
1180 CA PHE 172 0.0309 9.8686 7.4800
1191 CA ILE 173 0.0374 11.2476 9.2000
1199 CA PHE 174 0.0228 8.1509 13.1800
1210 CA ALA 175 0.0200 7.5765 12.5300
1215 CA ASN 176 0.0235 8.2994 17.5300
1223 CA VAL 177 0.0252 8.6706 23.5300
1230 CA SER 178 0.0476 13.3978 32.0500
1236 CA GLU 179 0.0535 14.6535 47.4800
1245 CA ALA 180 0.1269 30.1443 60.2600
1250 CA ASP 181 0.1644 38.0816 68.9000
1258 CA ASP 182 0.0978 23.9982 64.4100
1266 CA ARG 183 0.0550 14.9530 56.4300
1277 CA TYR 184 0.0364 11.0296 40.9800
1289 CA ILE 185 0.0318 10.0522 30.3500
1297 CA CYS 186 0.0218 7.9573 21.7400
1303 CA ASP 187 0.0301 9.7119 20.3700
1311 CA ARG 188 0.0260 8.8335 17.0900
1322 CA PHE 189 0.0325 10.2130 16.2800
1333 CA TYR 190 0.0500 13.9083 16.2900
1345 CA PRO 191 0.0953 23.4655 17.2700
1352 CA ASN 192 0.0795 20.1462 17.0400
1360 CA ASP 193 0.0534 14.6213 17.0000
1368 CA LEU 194 0.0521 14.3489 15.1200
1376 CA TRP 195 0.0368 11.1250 14.8600
1390 CA VAL 196 0.0291 9.4940 12.4100
1397 CA VAL 197 0.0273 9.1036 10.2500
1404 CA VAL 198 0.0323 10.1660 9.8400
1411 CA PHE 199 0.0265 8.9423 10.8400
1422 CA GLN 200 0.0230 8.2084 7.9700
1431 CA PHE 201 0.0279 9.2368 6.2500
1442 CA GLN 202 0.0270 9.0584 5.4900
1451 CA HIS 203 0.0260 8.8349 3.6300
1461 CA ILE 204 0.0220 8.0018 3.3100
1469 CA MET 205 0.0260 8.8450 2.8400
1477 CA VAL 206 0.0209 7.7630 3.0400
1484 CA GLY 207 0.0181 7.1783 1.7200
1488 CA LEU 208 0.0183 7.2002 0.7200
1496 CA ILE 209 0.0251 8.6421 0.4800
1504 CA LEU 210 0.0297 9.6226 0.0600
1512 CA PRO 211 0.0195 7.4535 0.2700
1519 CA GLY 212 0.0243 8.4842 0.9600
1523 CA ILE 213 0.0421 12.2313 0.9600
1531 CA VAL 214 0.0247 8.5695 1.0000
1538 CA ILE 215 0.0161 6.7533 1.3100
1546 CA LEU 216 0.0357 10.8891 1.8500
1554 CA SER 217 0.0417 12.1454 2.8400
1560 CA CYS 218 0.0217 7.9329 4.8800
1566 CA TYR 219 0.0223 8.0475 5.8200
1578 CA CYS 220 0.0539 14.7262 4.9400
1584 CA ILE 221 0.0495 13.8010 5.1400
1592 CA ILE 222 0.0306 9.8112 7.8200
1600 CA ILE 223 0.0579 15.5738 8.1100
1608 CA SER 224 0.0821 20.6889 8.3100
1614 CA LYS 225 0.0637 16.7920 11.8100
1623 CA LEU 226 0.0734 18.8438 20.1400
1631 CA SER 227 0.1492 34.8553 33.5500
1637 CA HIS 228 0.1586 36.8441 49.2300
1647 CA SER 229 0.2266 51.2128 49.8200
1653 CA LYS 230 0.2489 55.9132 49.0900
1662 CA GLY 231 0.2437 54.8255 51.5100
1666 CA HIS 232 0.1670 38.6257 51.3900
1676 CA GLN 233 0.1477 34.5395 47.4400
1685 CA LYS 234 0.0758 19.3649 40.6800
1694 CA ARG 235 0.0736 18.8873 42.9300
1705 CA LYS 236 0.0958 23.5766 39.2300
1714 CA ALA 237 0.0675 17.6079 31.8200
1719 CA LEU 238 0.0270 9.0496 20.8900
1727 CA LYS 239 0.0471 13.2905 15.6200
1736 CA THR 240 0.0266 8.9540 11.9200
1743 CA THR 241 0.0166 6.8457 11.1100
1750 CA VAL 242 0.0186 7.2732 9.4500
1757 CA ILE 243 0.0245 8.5109 8.8700
1765 CA LEU 244 0.0143 6.3750 6.7600
1773 CA ILE 245 0.0138 6.2501 5.1300
1781 CA LEU 246 0.0234 8.2809 5.4900
1789 CA ALA 247 0.0199 7.5476 4.3900
1794 CA PHE 248 0.0128 6.0500 3.0000
1805 CA PHE 249 0.0140 6.3053 2.7700
1816 CA ALA 250 0.0172 6.9770 3.3900
1821 CA CYS 251 0.0152 6.5600 4.6500
1827 CA TRP 252 0.0165 6.8195 2.1300
1841 CA LEU 253 0.0192 7.4025 1.9400
1849 CA PRO 254 0.0205 7.6781 1.3800
1856 CA TYR 255 0.0179 7.1336 1.4600
1868 CA TYR 256 0.0183 7.2029 0.9100
1880 CA ILE 257 0.0238 8.3790 1.0900
1888 CA GLY 258 0.0207 7.7137 2.2600
1892 CA ILE 259 0.0207 7.7094 2.0100
1900 CA SER 260 0.0270 9.0582 2.3500
1906 CA ILE 261 0.0266 8.9614 3.8200
1914 CA ASP 262 0.0227 8.1340 5.5600
1922 CA SER 263 0.0261 8.8534 5.6800
1928 CA PHE 264 0.0408 11.9655 6.1200
1939 CA ILE 265 0.0340 10.5359 8.2000
1947 CA LEU 266 0.0407 11.9520 9.0100
1955 CA LEU 267 0.0603 16.0720 8.8900
1963 CA GLU 268 0.0948 23.3693 12.5400
1972 CA ILE 269 0.0848 21.2612 10.3400
1980 CA ILE 270 0.0647 17.0095 12.6100
1988 CA LYS 271 0.0865 21.6144 20.0800
1997 CA GLN 272 0.0969 23.8198 23.1000
2006 CA GLY 273 0.1240 29.5459 28.7800
2010 CA CYS 274 0.0916 22.6861 34.7100
2016 CA GLU 275 0.0809 20.4362 31.7200
2025 CA PHE 276 0.0439 12.6100 21.9000
2036 CA GLU 277 0.0344 10.6153 22.0300
2045 CA ASN 278 0.0374 11.2484 20.5100
2053 CA THR 279 0.0367 11.0879 14.4000
2060 CA VAL 280 0.0252 8.6678 11.6100
2067 CA HIS 281 0.0294 9.5646 10.2200
2077 CA LYS 282 0.0287 9.4162 7.7400
2086 CA TRP 283 0.0223 8.0456 3.8800
2100 CA ILE 284 0.0206 7.6857 3.9500
2108 CA SER 285 0.0286 9.3913 3.8300
2114 CA ILE 286 0.0241 8.4387 3.5500
2122 CA THR 287 0.0209 7.7556 2.7900
2129 CA GLU 288 0.0191 7.3789 3.7800
2138 CA ALA 289 0.0200 7.5785 2.6800
2143 CA LEU 290 0.0167 6.8779 4.2300
2151 CA ALA 291 0.0154 6.5981 3.6200
2156 CA PHE 292 0.0124 5.9699 2.6000
2167 CA PHE 293 0.0133 6.1544 4.8800
2178 CA HIS 294 0.0122 5.9317 5.6800
2188 CA CYS 295 0.0119 5.8657 4.8600
2194 CA CYS 296 0.0133 6.1565 6.1700
2200 CA LEU 297 0.0148 6.4736 11.8500
2208 CA ASN 298 0.0140 6.3089 9.4400
2216 CA PRO 299 0.0171 6.9573 9.5700
2223 CA ILE 300 0.0263 8.8983 9.0000
2231 CA LEU 301 0.0250 8.6294 10.3200
2239 CA TYR 302 0.0313 9.9603 11.1900
2251 CA ALA 303 0.0661 17.3099 11.5200
2256 CA PHE 304 0.1210 28.9014 14.4900
2267 CA LEU 305 0.1719 39.6573 14.6100

If you find results from this site helpful for your research, please cite one of our papers:

K. Suhre & Y.H. Sanejouand, ElNemo: a normal mode web-server for protein movement analysis and the generation of templates for molecular replacement. Nucleic Acids Research, 32, W610-W614, 2004.
K. Suhre & Y.H. Sanejouand, On the potential of normal mode analysis for solving difficult molecular replacement problems. Acta Cryst. D vol.60, p796-799, 2004 © International Union of Crystallography.

elNémo is maintained by Yves-Henri Sanejouand.
It was developed by Karsten Suhre.
Between 2003 and 2014, it was hosted by IGS (Marseille).
Between 2015 and 2025, it was hosted by US2B (Nantes).
Last modification: december 26th, 2025.

Should you encounter any unexpected behaviour, please let us know. elNémo has been hacked on november 27th. It has been moved away and runs again. **Still some additional cleaning from time to time** Sorry for the inconvenience.