Should you encounter any unexpected behaviour,
please let us know.
elNémo has been hacked on november 27th.
It has been moved away and runs again.
Still some additional cleaning from time to time
Sorry for the inconvenience.

* TRANSPORT PROTEIN 23-FEB-99 1NGL *

B-factor analysis for ID 2604161210002404095

Overall B-factor prediction

Correlation= 0.884 for 179 C-alpha atoms.

B-factors for all residues

Residue-by-residue B-factor prediction for CA atoms

B-factors are computed based on the first 100 normal modes (B-PREDICT) and are scaled to match the overall B-factors in the submitted model (B-SCALED). A high correlation with the observations is an indicator that the normal modes capture well the overall flexibility features of the model. Comparison of the observed (B-OBS, blue) and the computed (B-SCALED, red) B-factors for all C-alpha atoms is presented below. Differences between B-factors from normal mode analysis and from X-ray crystallographic data may for example indicate regions where the protein flexibility is modified due to external factors such as tight crystall-packing.

ATOM TYP RES RESID B-PREDICT B-SCALED B-OBS	B-SCALED	B-OBS
2 CA MET 1 1.9706 17.3905 14.9600
21 CA GLN 2 2.0917 18.4037 13.9000
38 CA ASP 3 1.4475 13.0135 12.0000
50 CA SER 4 0.9067 8.4874 9.7900
61 CA THR 5 0.4704 4.8372 8.1800
75 CA SER 6 0.2089 2.6486 6.0600
86 CA ASP 7 0.1564 2.2091 4.7200
98 CA LEU 8 0.0972 1.7138 3.8300
117 CA ILE 9 0.0464 1.2890 3.0600
136 CA PRO 10 0.0821 1.5879 3.6100
150 CA ALA 11 0.0772 1.5470 3.2300
160 CA PRO 12 0.0340 1.1856 2.3600
174 CA PRO 13 0.0383 1.2213 3.2800
188 CA LEU 14 0.0262 1.1200 2.6100
207 CA SER 15 0.0209 1.0759 3.2900
218 CA LYS 16 0.0141 1.0185 2.2600
240 CA VAL 17 0.0116 0.9977 1.0400
256 CA PRO 18 0.0096 0.9809 0.9100
270 CA LEU 19 0.0061 0.9518 0.8500
289 CA GLN 20 0.0047 0.9404 0.9100
306 CA GLN 21 0.0126 1.0065 0.9900
323 CA ASN 22 0.0142 1.0195 1.2600
337 CA PHE 23 0.0073 0.9617 1.2200
357 CA GLN 24 0.0105 0.9889 1.0300
374 CA ASP 25 0.0080 0.9682 0.8700
386 CA ASN 26 0.0149 1.0252 0.8400
400 CA GLN 27 0.0133 1.0123 0.9600
417 CA PHE 28 0.0056 0.9475 0.8500
437 CA GLN 29 0.0062 0.9525 0.4900
454 CA GLY 30 0.0053 0.9449 0.4700
461 CA LYS 31 0.0041 0.9353 0.3900
483 CA TRP 32 0.0028 0.9241 0.3800
507 CA TYR 33 0.0035 0.9301 0.4100
528 CA VAL 34 0.0033 0.9284 0.4100
544 CA VAL 35 0.0051 0.9432 0.3800
560 CA GLY 36 0.0065 0.9550 0.4800
567 CA LEU 37 0.0074 0.9626 0.6600
586 CA ALA 38 0.0100 0.9849 0.5600
596 CA GLY 39 0.0126 1.0063 0.4300
603 CA ASN 40 0.0194 1.0631 0.7000
617 CA ALA 41 0.0199 1.0672 0.8900
627 CA ILE 42 0.0153 1.0290 0.8100
646 CA LEU 43 0.0228 1.0919 1.2600
665 CA ARG 44 0.0193 1.0622 1.3900
689 CA GLU 45 0.0344 1.1886 2.1400
704 CA ASP 46 0.0512 1.3291 3.4900
716 CA LYS 47 0.0724 1.5062 4.4000
738 CA ASP 48 0.0435 1.2652 3.7400
750 CA PRO 49 0.0246 1.1064 3.6800
764 CA GLN 50 0.0126 1.0063 3.3100
781 CA LYS 51 0.0111 0.9938 2.1000
803 CA MET 52 0.0064 0.9547 1.2300
820 CA TYR 53 0.0043 0.9366 0.6300
841 CA ALA 54 0.0032 0.9279 0.3700
851 CA THR 55 0.0028 0.9240 0.2900
865 CA ILE 56 0.0040 0.9341 0.3000
884 CA TYR 57 0.0036 0.9308 0.3500
905 CA GLU 58 0.0074 0.9630 0.3400
920 CA LEU 59 0.0100 0.9843 0.4000
939 CA LYS 60 0.0194 1.0628 0.5600
961 CA GLU 61 0.0396 1.2319 0.7500
976 CA ASP 62 0.0285 1.1394 0.6500
988 CA LYS 63 0.0161 1.0358 0.6100
1010 CA SER 64 0.0107 0.9907 0.5000
1021 CA TYR 65 0.0070 0.9597 0.4300
1042 CA ASN 66 0.0083 0.9700 0.2900
1056 CA VAL 67 0.0051 0.9437 0.3300
1072 CA THR 68 0.0063 0.9531 0.2800
1086 CA SER 69 0.0052 0.9446 0.2700
1097 CA VAL 70 0.0058 0.9490 0.3900
1113 CA LEU 71 0.0089 0.9749 0.6600
1132 CA PHE 72 0.0109 0.9923 0.8500
1152 CA ARG 73 0.0235 1.0971 1.5200
1176 CA LYS 74 0.0407 1.2418 2.0200
1198 CA LYS 75 0.0385 1.2229 1.4200
1220 CA LYS 76 0.0267 1.1240 1.2600
1242 CA CYS 77 0.0130 1.0100 0.8300
1252 CA ASP 78 0.0153 1.0292 0.7500
1264 CA TYR 79 0.0119 1.0007 0.5600
1285 CA TRP 80 0.0129 1.0084 0.4900
1309 CA ILE 81 0.0106 0.9893 0.3500
1328 CA ARG 82 0.0088 0.9747 0.4000
1352 CA THR 83 0.0078 0.9661 0.3400
1366 CA PHE 84 0.0056 0.9474 0.3600
1386 CA VAL 85 0.0075 0.9632 0.3500
1402 CA PRO 86 0.0074 0.9629 0.3700
1416 CA GLY 87 0.0109 0.9923 0.4300
1423 CA CYS 88 0.0305 1.1563 0.5900
1434 CA GLN 89 0.0172 1.0447 0.7000
1451 CA PRO 90 0.0081 0.9685 0.7100
1465 CA GLY 91 0.0050 0.9430 0.6700
1472 CA GLU 92 0.0061 0.9521 0.4500
1487 CA PHE 93 0.0054 0.9463 0.3200
1507 CA THR 94 0.0074 0.9628 0.3000
1521 CA LEU 95 0.0081 0.9687 0.2900
1540 CA GLY 96 0.0137 1.0150 0.4200
1547 CA ASN 97 0.0241 1.1024 0.5000
1561 CA ILE 98 0.0211 1.0771 0.4800
1580 CA LYS 99 0.0432 1.2624 0.7600
1602 CA SER 100 0.0546 1.3580 0.9000
1613 CA TYR 101 0.0370 1.2106 0.7700
1634 CA PRO 102 0.0644 1.4395 0.8900
1648 CA GLY 103 0.0503 1.3217 0.7500
1655 CA LEU 104 0.0253 1.1121 0.5800
1674 CA THR 105 0.0269 1.1261 0.5700
1688 CA SER 106 0.0180 1.0512 0.4900
1699 CA TYR 107 0.0105 0.9890 0.4700
1720 CA LEU 108 0.0078 0.9662 0.4400
1739 CA VAL 109 0.0047 0.9398 0.4100
1755 CA ARG 110 0.0039 0.9337 0.4600
1779 CA VAL 111 0.0027 0.9230 0.4600
1795 CA VAL 112 0.0043 0.9372 0.4500
1811 CA SER 113 0.0045 0.9386 0.6000
1822 CA THR 114 0.0040 0.9345 0.5400
1836 CA ASN 115 0.0073 0.9618 0.6700
1850 CA TYR 116 0.0063 0.9536 0.7100
1871 CA ASN 117 0.0125 1.0058 0.6500
1885 CA GLN 118 0.0094 0.9792 0.5100
1902 CA HIS 119 0.0054 0.9457 0.3800
1919 CA ALA 120 0.0034 0.9290 0.3700
1929 CA MET 121 0.0036 0.9308 0.3600
1946 CA VAL 122 0.0030 0.9260 0.3300
1962 CA PHE 123 0.0052 0.9442 0.3800
1982 CA PHE 124 0.0056 0.9474 0.3600
2002 CA LYS 125 0.0093 0.9786 0.3700
2024 CA LYS 126 0.0122 1.0031 0.5600
2046 CA VAL 127 0.0207 1.0737 0.4900
2062 CA SER 128 0.0288 1.1417 0.5500
2073 CA GLN 129 0.0479 1.3018 0.6500
2090 CA ASN 130 0.0590 1.3942 0.7500
2104 CA ARG 131 0.0431 1.2612 0.7300
2128 CA GLU 132 0.0274 1.1300 0.6600
2143 CA TYR 133 0.0185 1.0557 0.6500
2164 CA PHE 134 0.0117 0.9986 0.5100
2184 CA LYS 135 0.0081 0.9690 0.4400
2206 CA ILE 136 0.0061 0.9522 0.4200
2225 CA THR 137 0.0043 0.9370 0.3600
2239 CA LEU 138 0.0041 0.9350 0.3500
2258 CA TYR 139 0.0031 0.9266 0.3500
2279 CA GLY 140 0.0043 0.9367 0.3600
2286 CA ARG 141 0.0045 0.9388 0.6200
2310 CA THR 142 0.0096 0.9814 0.6900
2324 CA LYS 143 0.0199 1.0676 0.9300
2346 CA GLU 144 0.0203 1.0703 1.1600
2361 CA LEU 145 0.0123 1.0035 0.6100
2380 CA THR 146 0.0189 1.0592 0.8100
2394 CA SER 147 0.0261 1.1190 1.3500
2405 CA GLU 148 0.0245 1.1055 1.4000
2420 CA LEU 149 0.0135 1.0137 0.9100
2439 CA LYS 150 0.0151 1.0273 1.2500
2461 CA GLU 151 0.0211 1.0774 1.5800
2476 CA ASN 152 0.0174 1.0466 1.4500
2490 CA PHE 153 0.0118 0.9992 1.2500
2510 CA ILE 154 0.0166 1.0399 1.3500
2529 CA ARG 155 0.0224 1.0879 1.3900
2553 CA PHE 156 0.0171 1.0438 1.2700
2573 CA SER 157 0.0149 1.0254 1.2600
2584 CA LYS 158 0.0212 1.0781 1.3000
2606 CA SER 159 0.0240 1.1016 1.4100
2617 CA LEU 160 0.0212 1.0779 1.6000
2636 CA GLY 161 0.0200 1.0683 1.5200
2643 CA LEU 162 0.0252 1.1118 1.1700
2662 CA PRO 163 0.0277 1.1326 1.1500
2676 CA GLU 164 0.0286 1.1401 1.0400
2691 CA ASN 165 0.0237 1.0989 0.9700
2705 CA HIS 166 0.0186 1.0567 0.7200
2722 CA ILE 167 0.0145 1.0224 0.5400
2741 CA VAL 168 0.0138 1.0160 0.6000
2757 CA PHE 169 0.0108 0.9916 0.6300
2777 CA PRO 170 0.0121 1.0022 1.0000
2791 CA VAL 171 0.0086 0.9724 1.0300
2807 CA PRO 172 0.0124 1.0050 1.9000
2821 CA ILE 173 0.0135 1.0137 2.8000
2840 CA ASP 174 0.0130 1.0093 2.7900
2852 CA GLN 175 0.0109 0.9923 2.1400
2869 CA CYS 176 0.0106 0.9898 1.3700
2879 CA ILE 177 0.0210 1.0764 2.1400
2898 CA ASP 178 0.0319 1.1677 3.4400
2910 CA GLY 179 0.0382 1.2203 4.9100

If you find results from this site helpful for your research, please cite one of our papers:

K. Suhre & Y.H. Sanejouand, ElNemo: a normal mode web-server for protein movement analysis and the generation of templates for molecular replacement. Nucleic Acids Research, 32, W610-W614, 2004.
K. Suhre & Y.H. Sanejouand, On the potential of normal mode analysis for solving difficult molecular replacement problems. Acta Cryst. D vol.60, p796-799, 2004 © International Union of Crystallography.

elNémo is maintained by Yves-Henri Sanejouand.
It was developed by Karsten Suhre.
Between 2003 and 2014, it was hosted by IGS (Marseille).
Between 2015 and 2025, it was hosted by US2B (Nantes).
Last modification: december 26th, 2025.

Should you encounter any unexpected behaviour, please let us know. elNémo has been hacked on november 27th. It has been moved away and runs again. **Still some additional cleaning from time to time** Sorry for the inconvenience.